IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chauhan, B. K.
Right arrow Articles by Cvekl, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chauhan, B. K.
Right arrow Articles by Cvekl, A.
(Investigative Ophthalmology and Visual Science. 2002;43:1884-1890.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

Identification of Differentially Expressed Genes in Mouse Pax6 Heterozygous Lenses

Bharesh K. Chauhan1,2, Weiyan Zhang2,3, Kveta Cveklova1, Marc Kantorow3 and Ales Cvekl1

1 From the Departments of Ophthalmology and Visual Sciences and Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York; and the 3 Department of Biology, West Virginia University, Morgantown, West Virginia.

PURPOSE. Pax6 is a critical regulator of the developing lens, other ocular tissues, central nervous system, and pancreas. Downstream targets of Pax6 are largely unknown. The present study was designed to identify differentially expressed genes in Pax6 heterozygous and normal mouse lenses.

METHODS. RNAs from 8-week-old normal and Pax6 heterozygous mouse lenses were analyzed by both RT-PCR differential display and a candidate-gene approach. The expression levels of identified genes were confirmed by semiquantitative RT-PCR.

RESULTS. Eight transcripts encoding phosphatase inhibitor Pip-1 protein, heat shock protein Hsp40, Purkinje cell protein Pcp4, an expressed sequence tag (EST; AA331381) originally reported in a brain-specific library, Pitx3, and CBP, were confirmed to be downregulated in the Pax6 heterozygous mouse lenses. {alpha}B- and ßA3/A1-crystallin transcripts exhibited decreased expression in Pax6 heterozygous lenses, whereas the expression levels of other crystallins were virtually unchanged.

CONCLUSIONS. The present data identify eight genes with expression levels that are decreased in Pax6 heterozygous lenses and provide evidence that four functional categories of transcripts—namely, small hsps ({alpha}B-crystallin and Hsp40), crystallins ({alpha}B- and ßA3/A1-crystallin), transcription factors (Pitx-3 and CBP), and components of signal transduction cascades (Pip-1) are under direct or indirect transcriptional control by Pax6.




This article has been cited by other articles:


Home page
 Stem CellsHome page
C. M. Ferrell, S. T. Dorsam, H. Ohta, R. K. Humphries, M. K. Derynck, C. Haqq, C. Largman, and H. J. Lawrence
Activation of Stem-Cell Specific Genes by HOXA9 and HOXA10 Homeodomain Proteins in CD34+ Human Cord Blood Cells
Stem Cells, May 1, 2005; 23(5): 644 - 655.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Zaniolo, S. Leclerc, A. Cvekl, L. Vallieres, R. Bazin, K. Larouche, and S. L. Guerin
Expression of the {alpha}4 Integrin Subunit Gene Promoter Is Modulated by the Transcription Factor Pax-6 in Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1692 - 1704.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. K. Chauhan, Y. Yang, K. Cveklova, and A. Cvekl
Functional interactions between alternatively spliced forms of Pax6 in crystallin gene regulation and in haploinsufficiency
Nucleic Acids Res., March 12, 2004; 32(5): 1696 - 1709.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Association for Research in Vision and Ophthalmology