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(Investigative Ophthalmology and Visual Science. 2002;43:2317-2325.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

P2 Purinergic Receptor–Coupled Signaling in the Rabbit Ciliary Body Epithelium

Nasser A. Farahbakhsh and Marianne C. Cilluffo

From the Department of Physiological Science, University of California, Los Angeles, California.

PURPOSE. To identify and characterize P2 purinergic receptors and their signaling pathways in the epithelial cells of the rabbit ciliary body.

METHODS. Real-time fluorescence ratio imaging of the intact fura-2-loaded nonpigmented ciliary body epithelial (NPE) cells of rabbit were used to record changes in the intracellular free calcium concentration ([Ca2+]i), in response to a number of purinergic agonists and antagonists. The effects of some of these drugs on the inositol phosphate (IP) levels in ciliary processes were also examined.

RESULTS. Adenosine diphosphate (ADP), adenosine triphosphate (ATP), and uridine triphosphate (UTP) dose dependently increased the [Ca2+]i and IP levels. The [Ca2+]i increases induced by ADP and UTP were distinguishable, both kinetically and pharmacologically. The effect of ADP on [Ca2+]i was mimicked by a number of P2Y1-selective agonists, and was blocked by three P2Y1-receptor-specific antagonists. The [Ca2+]i increases elicited by ADP (or its analogs) and UTP were additive.

CONCLUSIONS. Rabbit ciliary body epithelium possesses both P2Y1 and P2Y2 metabotropic purinergic receptor subtypes, which differentially use the IP3/Ca2+ second-messenger pathway.




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Copyright © 2002 by the Association for Research in Vision and Ophthalmology