IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van den Aardweg, G. J. M. J.
Right arrow Articles by Luyten, G. P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van den Aardweg, G. J. M. J.
Right arrow Articles by Luyten, G. P. M.
(Investigative Ophthalmology and Visual Science. 2002;43:2561-2565.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

Cellular Radiosensitivity of Primary and Metastatic Human Uveal Melanoma Cell Lines

Gerard J. M. J. van den Aardweg1, Nicole C. Naus2,3, Anette C. A. Verhoeven2,3, Annelies de Klein4 and Gregorius P. M. Luyten2

1 From the Department of Radiation Oncology, Division of Clinical Radiobiology, and the 2 Departments of Ophthalmology, 3 Cell Biology and Genetics, and 4 Clinical Genetics, University Hospital Rotterdam, Rotterdam, The Netherlands.

PURPOSE. To investigate the radiosensitivity of uveal melanoma cell lines by a clonogenic survival assay, to improve the efficiency of the radiation regimen.

METHODS. Four primary and four metastatic human uveal melanoma cell lines were cultured in the presence of conditioned medium. After single-dose irradiation (0–12 Gy), colonies were allowed to form for 6 to 14 days. Two cutaneous melanomas cell lines were also tested for comparison. The survival curves were analyzed by the linear quadratic (LQ) model, and the surviving fraction at a dose of 2 Gy (SF2), the SF at 10 Gy (SF10), the ratio of initial irreparably damaged DNA ({alpha}-coefficient) to the capacity to repair sublethally damaged DNA (ß-coefficient), and the plating efficiency were calculated.

RESULTS. The melanomas displayed a wide range of initial irreparable DNA damage ({alpha}-component), as well as a capacity for repair of sublethal DNA damage (ß-component), which ultimately resulted in a wide range of {alpha}/ß ratios. These findings were similar in both primary and metastatic melanomas and were comparable with data obtained from two cutaneous melanomas.

CONCLUSIONS. Cell lines obtained from primary and metastatic human uveal melanomas displayed a wide range of radiosensitivity, similar to that published for cutaneous melanomas. Translating these data to the clinical setting indicates that a fractionated dose of 8 to 10 Gy administered in three to four fractions, as currently delivered in many centers, should be sufficient to eradicate tumors of approximately 1 cm3.




This article has been cited by other articles:


Home page
 Arch OphthalmolHome page
E. Kilic, T. Stijnen, P. T. V. M. de Jong, C. M. Mooy, W. M. H. Eijkenboom, P. J. Ringens, and G. P. M. Luyten
Reduced Melanoma-Related Mortality in Uveal Melanoma by Preenucleation Radiotherapy
Arch Ophthalmol, October 1, 2005; 123(10): 1363 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. J. M. J. van den Aardweg, E. Kilic, A. de Klein, and G. P. M. Luyten
Dose Fractionation Effects in Primary and Metastatic Human Uveal Melanoma Cell Lines
Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4660 - 4664.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Association for Research in Vision and Ophthalmology