| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1From the Department of Zoology and Genetics and the 2Neuroscience Program, Iowa State University, Ames, Iowa; and the 3Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts.
PURPOSE. To investigate the influence of a developing host environment on the survival, differentiation, and morphologic integration of murine brain progenitor cells (mBPCs) transplanted into the mammalian retina.
METHODS. Enhanced green fluorescent protein (GFP)–expressing murine brain progenitor cells were transplanted into developing and mature Brazilian opossums (Monodelphis domestica). Animals were allowed to survive for up to 4 weeks after transplantation, at which time the eyes were prepared for immunohistochemical analysis.
RESULTS. Transplanted mBPCs survived and differentiated in vivo, and extensive morphologic integration was observed within the host retinas. GFP-expressing cells often displayed morphologies characteristic of retinal neurons. GFP somata were located in nuclear layers, and their processes ramified throughout the inner (IPL) and outer (OPL) plexiform layers. Furthermore, in some cases, GFP-expressing neurites were confined to specific sublamina within the IPL. The greatest morphologic integration and differentiation were observed after transplantation into the youngest-aged host eyes. Some transplanted mBPCs incorporated within the inner retina expressed the neuronal markers microtubule associated protein (MAP)-2 or calretinin. Transplanted cells coexpressed GFP and recoverin only in the ONL.
CONCLUSIONS. mBPCs survived and morphologically integrated after xenotransplantation without immunosuppression. mBPCs were capable of incorporating into specific layers of the retina and expressing neuronal and retinal markers. The age of the host appeared to play a key role in determining cell fate in vivo.
This article has been cited by other articles:
|
|
 |
|
  P. B. Samollow The opossum genome: Insights and opportunities from an alternative mammal Genome Res., August 1, 2008; 18(8): 1199 - 1215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Lawrence, S. Singhal, B. Bhatia, D. J. Keegan, T. A. Reh, P. J. Luthert, P. T. Khaw, and G. A. Limb MIO-M1 Cells and Similar Muller Glial Cell Lines Derived from Adult Human Retina Exhibit Neural Stem Cell Characteristics Stem Cells, August 1, 2007; 25(8): 2033 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Canola, B. Angenieux, M. Tekaya, A. Quiambao, M. I. Naash, F. L. Munier, D. F. Schorderet, and Y. Arsenijevic Retinal Stem Cells Transplanted into Models of Late Stages of Retinitis Pigmentosa Preferentially Adopt a Glial or a Retinal Ganglion Cell Fate Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 446 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, H. Klassen, M. Pries, W. Wang, and M. H. Nissen Aqueous Humor Enhances the Proliferation of Rat Retinal Precursor Cells in Culture, and This Effect Is Partially Reproduced by Ascorbic Acid Stem Cells, December 1, 2006; 24(12): 2766 - 2775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liang, R.-T. Yan, W. Ma, H. Zhang, and S.-Z. Wang Exploring RPE as a Source of Photoreceptors: Differentiation and Integration of Transdifferentiating Cells Grafted into Embryonic Chick Eyes Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 5066 - 5074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Meyer, M. L. Katz, J. A. Maruniak, and M. D. Kirk Embryonic Stem Cell-Derived Neural Progenitors Incorporate into Degenerating Retina and Enhance Survival of Host Photoreceptors Stem Cells, February 1, 2006; 24(2): 274 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Young, T. Borras, M. Walter, and R. Ritch Tissue Bioengineering: Potential Applications to Glaucoma Arch Ophthalmol, December 1, 2005; 123(12): 1725 - 1731. [Full Text] [PDF]
|
|
|
|
|
|
K. Warfvinge, J. F. Kiilgaard, E. B. Lavik, E. Scherfig, R. Langer, H. J. Klassen, and M. J. Young Retinal Progenitor Cell Xenografts to the Pig Retina: Morphologic Integration and Cytochemical Differentiation Arch Ophthalmol, October 1, 2005; 123(10): 1385 - 1393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Levin, R. Ritch, J. E. Richards, and T. Borras Stem Cell Therapy for Ocular Disorders Arch Ophthalmol, April 1, 2004; 122(4): 621 - 627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Wojciechowski, U. Englund, C. Lundberg, and K. Warfvinge Survival and Long Distance Migration of Brain-Derived Precursor Cells Transplanted to Adult Rat Retina Stem Cells, January 1, 2004; 22(1): 27 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R R Ali and J C Sowden Therapy may yet stem from cells in the retina Br. J. Ophthalmol., September 1, 2003; 87(9): 1058 - 1059. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
