| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1From the Program in Stem Cell Biology, University of Florida, Florida; and 2The Retina Center, Gainesville, Florida.
PURPOSE. Age-related macular degeneration (ARMD) is the primary cause of blindness in people aged of 50 years or more. The wet form leads to severe loss of central vision. Recent evidence supports that adult hematopoietic stem cells (HSCs) contribute to preretinal neovascularization. In the current study, it was determined whether HSCs, by producing both blood and blood vessels, provide functional hemangioblast activity during choroidal neovascularization (CNV) in mice.
METHODS. Gfp chimeric mice were developed by bone marrow ablation of C57BL/6J mice and reconstitution with donor tissue from gfp+/+ transgenic mice. Gfp chimeric mice underwent laser rupture of Bruch’s membrane and were killed and eyes enucleated at 1, 2, 3, and 4 weeks after laser injury. CNV was examined by confocal microscopy of retinal flatmounts. Because endothelial progenitor cells (EPCs) derive from HSCs, immunocytochemistry was used to quantify relative the EPC contribution to CNV.
RESULTS. Laser injury alone was sufficient to induce stem cell recruitment and subsequent CNV. Gfp+ cells formed part of the functional vasculature in the choroid as early as 1 week after injury and were present for the duration of the study. The relative EPC contribution to CNV remained fairly constant throughout the study and constituted almost 50% of the total vasculature.
CONCLUSIONS. Adult stem cells are recruited to the choroid in a model of CNV, where they contribute to forming aberrant new vessels. This observation suggests that targeting stem cell recruitment to the eye may offer a novel therapeutic strategy for ARMD.
This article has been cited by other articles:
|
|
 |
|
  M. H. Criswell, W.-Z. Hu, T. J. Steffens, R. Li, and P. Margaron Comparing Pegaptanib and Triamcinolone Efficacy in the Rat Choroidal Neovascularization Model Arch Ophthalmol, July 1, 2008; 126(7): 946 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Thill, N. V. Strunnikova, M. J. Berna, N. Gordiyenko, K. Schmid, S. W. Cousins, D. J. S. Thompson, and K. G. Csaky Late Outgrowth Endothelial Progenitor Cells in Patients with Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2696 - 2708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Boettcher, E Ulbricht, D Helmlinger, A F Mack, A Reichenbach, P Wiedemann, H-J Wagner, M W Seeliger, A Bringmann, and J Priller Long-term engraftment of systemically transplanted, gene-modified bone marrow-derived cells in the adult mouse retina Br. J. Ophthalmol., February 1, 2008; 92(2): 272 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yodoi, M. Sasahara, T. Kameda, N. Yoshimura, and A. Otani Circulating Hematopoietic Stem Cells in Patients with Neovascular Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., December 1, 2007; 48(12): 5464 - 5472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Slayton, X.-M. Li, J. Butler, S. M. Guthrie, M. L. Jorgensen, J. R. Wingard, and E. W. Scott The Role of the Donor in the Repair of the Marrow Vascular Niche Following Hematopoietic Stem Cell Transplant Stem Cells, November 1, 2007; 25(11): 2945 - 2955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jost, C. Maillard, J. Lecomte, V. Lambert, M. Tjwa, P. Blaise, M.-L. Alvarez Gonzalez, K. Bajou, S. Blacher, P. Motte, et al. Tumoral and Choroidal Vascularization: Differential Cellular Mechanisms Involving Plasminogen Activator Inhibitor Type I Am. J. Pathol., October 1, 2007; 171(4): 1369 - 1380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I A Bhutto, D S McLeod, C Merges, T Hasegawa, and G A Lutty Localisation of SDF-1 and its receptor CXCR4 in retina and choroid of aged human eyes and in eyes with age related macular degeneration Br. J. Ophthalmol., July 1, 2006; 90(7): 906 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Sheridan, D. Rice, P. S. Hiscott, D. Wong, and D. L. Kent The Presence of AC133-Positive Cells Suggests a Possible Role of Endothelial Progenitor Cells in the Formation of Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1642 - 1645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Chan-Ling, L. Baxter, A. Afzal, N. Sengupta, S. Caballero, E. Rosinova, and M. B. Grant Hematopoietic Stem Cells Provide Repair Functions after Laser-Induced Bruch's Membrane Rupture Model of Choroidal Neovascularization Am. J. Pathol., March 1, 2006; 168(3): 1031 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sengupta, S. Caballero, R. N. Mames, A. M. Timmers, D. Saban, and M. B. Grant Preventing Stem Cell Incorporation into Choroidal Neovascularization by Targeting Homing and Attachment Factors Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 343 - 348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Dorrell, A. Otani, E. Aguilar, S. K. Moreno, and M. Friedlander Adult bone marrow-derived stem cells use R-cadherin to target sites of neovascularization in the developing retina Blood, May 1, 2004; 103(9): 3420 - 3427. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
