IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:4968-4975.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0140
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, F.
Right arrow Articles by Anderson, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, F.
Right arrow Articles by Anderson, R. E.

Alleviation of Constant-Light–Induced Photoreceptor Degeneration by Adaptation of Adult Albino Rat to Bright Cyclic Light

Feng Li,1,2 Wei Cao,1,2 and Robert E. Anderson1,2,3

1From the Departments of Ophthalmology and 2Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma; and the 3Dean A. McGee Eye Institute, Oklahoma City, Oklahoma.

PURPOSE. To further test the hypothesis that light-adaptation–mediated photoreceptor protection works through inhibition of apoptosis by activation and/or upregulation of neuroprotective molecules.

METHODS. Albino rats were born and raised in 5-lux cyclic light (12 hours OFF and ON). At 8 weeks of age, animals were adapted to 400-lux cyclic light for different periods. Light damage was induced by exposure to constant light for 1 day at an illumination of 1700 lux. Animals were killed, and their eyes were removed for morphometric and biochemical analysis. TUNEL assay was used to evaluate photoreceptor cell apoptosis and Western blot analyses were used to determine the levels of basic fibroblast growth factor (bFGF), neuronal nitric oxide synthase (nNOS), and caspase-3.

RESULTS. Exposure of dim-reared rats to constant light for 1 day dramatically increased TUNEL-positive cells in the outer nuclear layer. Adaptation to 400-lux bright cyclic light for 4 days significantly reduced TUNEL-positive cells induced by exposure to constant light, which correlated with a significant increase in bFGF expression. Compared with control retinas, caspase-3 levels were not changed by exposure to constant light or after adaptation to 400 lux. There was a significant increase in nNOS level in the constant-light–exposed group, but not in the group adapted to 400-lux bright light before exposure to constant light.

CONCLUSIONS. The retina of the adult rat can rapidly upregulate neuroprotective mechanisms when switched from dim to bright cyclic light. Identification of the molecules involved in this process may allow rational development of therapeutic approaches to treat retinal degenerative diseases.




This article has been cited by other articles:


Home page
 IOVSHome page
R. Maccarone, S. Di Marco, and S. Bisti
Saffron Supplement Maintains Morphology and Function after Exposure to Damaging Light in Mammalian Retina
Invest. Ophthalmol. Vis. Sci., March 1, 2008; 49(3): 1254 - 1261.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
O. Perche, M. Doly, and I. Ranchon-Cole
Caspase-Dependent Apoptosis in Light-Induced Retinal Degeneration
Invest. Ophthalmol. Vis. Sci., June 1, 2007; 48(6): 2753 - 2759.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Joly, V. Pernet, S. Chemtob, A. Di Polo, and P. Lachapelle
Neuroprotection in the Juvenile Rat Model of Light-Induced Retinopathy: Evidence Suggesting a Role for FGF-2 and CNTF
Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2311 - 2320.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Rajala, R. E. Anderson, J.-X. Ma, J. Lem, M. R. Al-Ubaidi, and R. V. S. Rajala
G-protein-coupled Receptor Rhodopsin Regulates the Phosphorylation of Retinal Insulin Receptor
J. Biol. Chem., March 30, 2007; 282(13): 9865 - 9873.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Li, R. E. Anderson, H. Tomita, R. Adler, X. Liu, D. J. Zack, and R. V. S. Rajala
Nonredundant Role of Akt2 for Neuroprotection of Rod Photoreceptor Cells from Light-Induced Cell Death
J. Neurosci., January 3, 2007; 27(1): 203 - 211.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Joly, V. Pernet, A. L. Dorfman, S. Chemtob, and P. Lachapelle
Light-induced retinopathy: comparing adult and juvenile rats.
Invest. Ophthalmol. Vis. Sci., July 1, 2006; 47(7): 3202 - 3212.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Rattner and J. Nathans
The Genomic Response to Retinal Disease and Injury: Evidence for Endothelin Signaling from Photoreceptors to Glia
J. Neurosci., May 4, 2005; 25(18): 4540 - 4549.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
R. Grewal, J. Stepczynski, R. Kelln, T. Erickson, R. Darrow, L. Barsalou, M. Patterson, D. T. Organisciak, and P. Wong
Coordinated Changes in Classes of Ribosomal Protein Gene Expression Is Associated with Light-Induced Retinal Degeneration
Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 3885 - 3895.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
H. Huang, F. Li, R. A. Alvarez, J. D. Ash, and R. E. Anderson
Downregulation of ATP Synthase Subunit-6, Cytochrome c Oxidase-III, and NADH Dehydrogenase-3 by Bright Cyclic Light in the Rat Retina
Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2489 - 2496.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. Zhang, B. Lei, T. T. Lam, F. Yang, D. Sinha, and M. O. M. Tso
Neuroprotection of Photoreceptors by Minocycline in Light-Induced Retinal Degeneration
Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2753 - 2759.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology