IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:5016-5022.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.03-0418
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, W.
Right arrow Articles by Busik, J. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, W.
Right arrow Articles by Busik, J. V.

Dyslipidemia, but Not Hyperglycemia, Induces Inflammatory Adhesion Molecules in Human Retinal Vascular Endothelial Cells

Weiqin Chen,1 Donald B. Jump,2 Maria B. Grant,3 Walter J. Esselman,1 and Julia V. Busik2

1From the Departments of Microbiology and Molecular Genetics and 2Physiology, Michigan State University, East Lansing, Michigan; and the 3Department of Pharmacology and Therapeutics, University of Florida, Gainesville, Florida.

PURPOSE. The initial determinants of retinal microvascular damage in diabetic retinopathy are not well understood, but are likely to be induced by hyperglycemia and/or dyslipidemia. The purpose of this study was to examine the effect of fatty acids and hyperglycemia on human retinal vascular endothelial (hRVE) cells as a means of mimicking diabetic metabolic disorders.

METHODS. The expression of adhesion molecules in hRVE and human umbilical vein endothelial cells (HUVECs) was assayed by Western blot analysis and confirmed by leukocyte adhesion assay. The mechanisms underlying the induction of adhesion molecules by fatty acids were further investigated by using cyclooxygenase (COX), lipoxygenase (LOX), and P450 monooxygenase (MOX) inhibitors.

RESULTS. Treatment of hRVE cells with the n6 polyunsaturated fatty acids (PUFAs) 18:2n6 and 20:4n6 for up to 24 hours resulted in a significant induction of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 protein levels. In contrast, treatment with high glucose (22 mM) for 24 hours did not affect CAM expression. Induction of CAM by n6 PUFA correlated with enhanced leukocyte binding to hRVE cells. The effect of n6 PUFA on ICAM-1 and VCAM-1 was blocked by an inhibitor of LOX, but not by COX or MOX inhibitors. In contrast to hRVE cells, n6 PUFA did not induce ICAM-1 or VCAM-1 in HUVECs.

CONCLUSIONS. The data obtained in this study demonstrate that acute exposure to linoleic or arachidonic acid, but not hyperglycemia, induces inflammatory adhesion molecule expression in the presence of LOX in microvascular hRVE cells, but not in HUVECs. These results are consistent with the emerging hypothesis recognizing early-stage diabetic retinopathy as a low-grade chronic inflammatory disease.




This article has been cited by other articles:


Home page
 DiabetesHome page
M. Tikhonenko, T. A. Lydic, Y. Wang, W. Chen, M. Opreanu, A. Sochacki, K. M. McSorley, R. L. Renis, T. Kern, D. B. Jump, et al.
Remodeling of Retinal Fatty Acids in an Animal Model of Diabetes: A Decrease in Long-Chain Polyunsaturated Fatty Acids Is Associated With a Decrease in Fatty Acid Elongases Elovl2 and Elovl4
Diabetes, January 1, 2010; 59(1): 219 - 227.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. V. Busik, S. Mohr, and M. B. Grant
Hyperglycemia-Induced Reactive Oxygen Species Toxicity to Endothelial Cells Is Dependent on Paracrine Mediators
Diabetes, July 1, 2008; 57(7): 1952 - 1965.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. E. Kanter, F. Johansson, R. C. LeBoeuf, and K. E. Bornfeldt
Do Glucose and Lipids Exert Independent Effects on Atherosclerotic Lesion Initiation or Progression to Advanced Plaques?
Circ. Res., March 30, 2007; 100(6): 769 - 781.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Chen, D. B. Jump, W. J. Esselman, and J. V. Busik
Inhibition of Cytokine Signaling in Human Retinal Endothelial Cells through Modification of Caveolae/Lipid Rafts by Docosahexaenoic Acid
Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 18 - 26.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Chen, W. J. Esselman, D. B. Jump, and J. V. Busik
Anti-inflammatory Effect of Docosahexaenoic Acid on Cytokine-Induced Adhesion Molecule Expression in Human Retinal Vascular Endothelial Cells
Invest. Ophthalmol. Vis. Sci., November 1, 2005; 46(11): 4342 - 4347.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. R. Barile, S. I. Pachydaki, S. R. Tari, S. E. Lee, C. M. Donmoyer, W. Ma, L. L. Rong, L. G. Buciarelli, T. Wendt, H. Horig, et al.
The RAGE Axis in Early Diabetic Retinopathy
Invest. Ophthalmol. Vis. Sci., August 1, 2005; 46(8): 2916 - 2924.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. Y. Kim, M. A. Johnson, D. S. McLeod, T. Alexander, B. C. Hansen, and G. A. Lutty
Neutrophils Are Associated With Capillary Closure in Spontaneously Diabetic Monkey Retinas
Diabetes, May 1, 2005; 54(5): 1534 - 1542.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. W. Kent Jr, A. G. Comuzzie, M. C. Mahaney, L. Almasy, D. L. Rainwater, J. L. VandeBerg, J. W. MacCluer, and J. Blangero
Intercellular Adhesion Molecule-1 Concentration Is Genetically Correlated With Insulin Resistance, Obesity, and HDL Concentration in Mexican Americans
Diabetes, October 1, 2004; 53(10): 2691 - 2695.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology