IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:5242-5251.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0768
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skelsey, M. E.
Right arrow Articles by Niederkorn, J. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skelsey, M. E.
Right arrow Articles by Niederkorn, J. Y.

Splenic B Cells Act as Antigen Presenting Cells for the Induction of Anterior Chamber-Associated Immune Deviation

Molly E. Skelsey,1 Elizabeth Mayhew,2 and Jerry Y. Niederkorn2

1From the Graduate Program in Immunology and the 2Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas.

PURPOSE. To characterize the role of B cells in the induction of anterior chamber-associated immune deviation (ACAID).

METHODS. An in vitro model of the ACAID spleen was used to recapitulate the events that occur when antigen is introduced into the anterior chamber of the eye and culminates in the appearance of antigen-specific, CD8+ suppressor cells.

RESULTS. In vitro–generated suppressor cells mimicked those produced by anterior chamber injection of antigen, as shown by their antigen specificity, surface expression of CD8, and capacity to suppress DTH, which is mediated by previously immunized T cells. B cells were found to be necessary for suppressor cell development. The B cell receptor (BCR) was necessary for the induction of ACAID and conveyed antigen specificity to the suppressor T cells. Lysosomal acidification of internalized antigen was necessary for B cells to induce ACAID; however, transporter of antigen processing (TAP) was not required for the generation of ACAID.

CONCLUSIONS. The results suggest that B cells use the BCR to capture and internalize antigen from ACAID-inducing macrophages. Lysosomal acidification of the captured antigen is essential for the processing of the ACAID antigen before TAP-independent presentation to suppressor cells.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
H. M. Ashour and T. M. Seif
The role of B cells in the induction of peripheral T cell tolerance
J. Leukoc. Biol., November 1, 2007; 82(5): 1033 - 1039.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
H. M. Ashour and J. Y. Niederkorn
{gamma}{delta} T Cells Promote Anterior Chamber-Associated Immune Deviation and Immune Privilege through Their Production of IL-10
J. Immunol., December 15, 2006; 177(12): 8331 - 8337.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. M. Ashour and J. Y. Niederkorn
Peripheral Tolerance Via the Anterior Chamber of the Eye: Role of B Cells in MHC Class I and II Antigen Presentation
J. Immunol., May 15, 2006; 176(10): 5950 - 5957.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
H. Keino, S. Masli, S. Sasaki, J. W. Streilein, and J. Stein-Streilein
CD8+ T Regulatory Cells Use a Novel Genetic Program that Includes CD103 to Suppress Th1 Immunity in Eye-Derived Tolerance.
Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1533 - 1542.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology