IOVS AJP: Regulatory, Integrative and Comparative Physiology
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(Investigative Ophthalmology and Visual Science. 2003;44:573-580.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.01-1185
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Overexpression of Insulin-like Growth Factor-Binding Protein-2 in Pterygium Body Fibroblasts

Abraham Solomon,1 Martin Grueterich,1 De-Quan Li,1 Daniel Meller,2 Sao-Bing Lee,3 and Scheffer C. G. Tseng1,4

1From the Department of Ophthalmology, Bascom Palmer Eye Institute; the 2Department of Ophthalmology, University of Essen, Essen, Germany; the 3Singapore National Eye Center, Singapore; and the 4Department of Cell Biology and Anatomy, University of Miami School of Medicine, Miami, Florida.

PURPOSE. To examine the expression pattern of insulin-like growth factor-binding protein (IGFBP)-2 in cultured primary pterygium fibroblasts and compare it with expression in normal conjunctival fibroblasts.

METHODS. Profile of gene expression by normal conjunctival and primary pterygium fibroblasts was performed by using a cDNA microarray. The overexpression of IGFBP-2 thus identified was further confirmed by RT-PCR and Western blot analysis of cultured cells and by immunohistochemistry on primary pterygium and normal conjunctival tissue sections.

RESULTS. A dramatically increased expression of IGFBP-2 mRNA was demonstrated in cDNA microarray membranes from two different pterygium fibroblasts. This finding was confirmed by RT-PCR in four additional different pterygium fibroblasts and by Western blot analysis of their culture supernatants. Immunohistochemistry of frozen sections from primary pterygium demonstrated increased staining in extracellular matrix of the stroma, compared with that of the normal conjunctiva. IGFBP-2 was also found in goblet cells of both normal conjunctival and pterygium epithelia.

CONCLUSIONS. The increased expression of IGFBP-2 mRNA and protein in pterygium fibroblasts is further strong evidence to support the transformed phenotype of these cells and helps explain why there is increased growth of fibrovascular tissue. This phenotype may be used as a marker to assess the malignant nature of pterygium growth and recurrence.




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