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(Investigative Ophthalmology and Visual Science. 2003;44:799-807.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.02-0267
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Non-neural Regions of the Adult Human Eye: A Potential Source of Neurons?

Yvan Arsenijevic,1 Nicole Taverney,1 Corinne Kostic,1 Meriem Tekaya,1 François Riva,1 Leonidas Zografos,2 Daniel Schorderet,2 and Francis Munier1,3

1From the Unit of Oculogenetics and the 2Department of Ophthalmology, Jules Gonin Eye Hospital, Lausanne University, Lausanne, Switzerland; and the 3Division of Genetic Medicine, Lausanne University Medical School, Lausanne, Switzerland.

PURPOSE. Because it is known that both melanocytes and neurons are generated from neural crest stem cells and their derived precursors, the current study was undertaken to evaluate whether adult human ocular tissues, containing melanocytes, have the capacity to generate neuronlike cells in vitro.

METHODS. Choroid and Sclera cells from adult human eyes were separately dissociated and cultivated in the presence of epidermal growth factor and 10% fetal bovine serum. No retinal pigmented epithelial cells were detected. After cell growth, cells were transferred under conditions known to induce neuronal differentiation. Cells were plated on laminin in the presence of fibroblast growth factor-2 or brain-derived neurotrophic factor.

RESULTS. Cells derived from the sclera and the choroid of 15 donors proliferated to attain a 108-fold increase in the number of cells within 4 months. At each passage, groups of cells differentiated into cells with neuronal morphology, expressing neuronal markers confirmed by immunocytochemistry and RT-PCR analyses, such as ß-tubulin-III, neurofilament, and tau. Parallel to neuronlike formation, glialike cells, revealed by expression of vimentin and P0, were generated in large amounts. Although, absent from choroid and sclera tissues, nondifferentiated cells appeared in cultures.

CONCLUSIONS. The adult human eye conserves cells able to recapitulate certain neural developmental features. This observation opens new perspectives to study human neurogenesis and to provide an important source of neurons for transplantation studies in the retina and other regions of the central nervous system.




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E J Mayer, D A Carter, Y Ren, E H Hughes, C M Rice, C A Halfpenny, N J Scolding, and A D Dick
Neural progenitor cells from postmortem adult human retina
Br. J. Ophthalmol., January 1, 2005; 89(1): 102 - 106.
[Abstract] [Full Text] [PDF]


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