| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1From the Department of Ophthalmology, Mt. Sinai School of Medicine, New York, New York; and the 2Department of Ophthalmology, University Central Hospital, Helsinki, Finland.
PURPOSE. To evaluate the applicability of rebound tonometry for measurement of IOP in the mouse eye.
METHODS. An induction–impact (I/I) tonometer, which operates on the rebound principle, was scaled down and adapted to determine IOP of the mouse eye. IOP measurement using this concept is based on contacting the eye with a probe and detecting the motion as the probe collides with the eye and bounces back. The motion parameters of the probe vary according to eye pressure and are used to calculate IOP. A prototype instrument was constructed for measurement of IOP in mouse eyes, and its ability to accurately and reliably measure IOP was tested by comparing the measurements against the manometric (true) IOP determined in cannulated mouse eyes ex vivo. The I/I tonometer was also used to measure IOP in vivo in anesthetized adult C57BL/6 mice.
RESULTS. A strong correlation between the true IOP and the I/I measurements (R2 = 0.95) was found for IOPs in the range of 3.7 to 44.1 mm Hg in cannulated mouse eyes. Repeat determinations in individual eyes showed a low degree of variability in the relationship of the measured IOP with the true IOP. In anesthetized mice, mean IOP ± SD as determined by rebound tonometry was 9.8 ± 3.9 mm Hg when the animals were anesthetized with ketamine alone and 7.6 ± 1.9 mm Hg when a mixture of ketamine, acepromazine, and xylazine was used. Contralateral eyes differed by 0.9 ± 2.5 and 0.1 ± 2.7 mm Hg, respectively, for the two anesthetic regimens.
CONCLUSIONS. The I/I tonometer can be used for noninvasive, in vivo IOP measurement in mouse eyes. The availability of an easy-to-use, reliable tonometer for IOP measurements in mice will allow more extensive use of the mouse as a model for glaucoma.
This article has been cited by other articles:
|
|
 |
|
  M. Saleh, M. Nagaraju, and V. Porciatti Longitudinal Evaluation of Retinal Ganglion Cell Function and IOP in the DBA/2J Mouse Model of Glaucoma Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4564 - 4572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nagaraju, M. Saleh, and V. Porciatti IOP-Dependent Retinal Ganglion Cell Dysfunction in Glaucomatous DBA/2J Mice Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4573 - 4579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Crowston, C. A. Morris, J. D. Lindsey, and R. N. Weinreb Prostaglandin FP Receptors Do Not Contribute to 24-hour Intraocular Pressure Variation in Mice Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2095 - 2098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. P. Kocaoglu, S. R. Uhlhorn, E. Hernandez, R. A. Juarez, R. Will, J.-M. Parel, and F. Manns Simultaneous Fundus Imaging and Optical Coherence Tomography of the Mouse Retina Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 1283 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Cervino Rebound tonometry: new opportunities and limitations of non-invasive determination of intraocular pressure. Br. J. Ophthalmol., December 1, 2006; 90(12): 1444 - 1446. [Full Text] [PDF]
|
|
|
|
|
|
A. Maeda, S. Tsujiya, T. Higashide, K. Toida, T. Todo, T. Ueyama, H. Okamura, and K. Sugiyama Circadian intraocular pressure rhythm is generated by clock genes. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 4050 - 4052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M E Iliev, D Goldblum, K Katsoulis, C Amstutz, and B Frueh Comparison of rebound tonometry with Goldmann applanation tonometry and correlation with central corneal thickness Br. J. Ophthalmol., July 1, 2006; 90(7): 833 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Inman, R. M. Sappington, P. J. Horner, and D. J. Calkins Quantitative Correlation of Optic Nerve Pathology with Ocular Pressure and Corneal Thickness in the DBA/2 Mouse Model of Glaucoma. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 986 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Crowston, J. D. Lindsey, C. A. Morris, L. Wheeler, F. A. Medeiros, and R. N. Weinreb Effect of Bimatoprost on Intraocular Pressure in Prostaglandin FP Receptor Knockout Mice Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4571 - 4577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Martinez-de-la-Casa, J. Garcia-Feijoo, A. Castillo, and J. Garcia-Sanchez Reproducibility and Clinical Evaluation of Rebound Tonometry Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4578 - 4580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou, F. Li, L. Kong, H. Tomita, C. Li, and W. Cao Involvement of Inflammation, Degradation, and Apoptosis in a Mouse Model of Glaucoma J. Biol. Chem., September 2, 2005; 280(35): 31240 - 31248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Avila, A. Munera, A. Guzman, C. W. Do, Z. Wang, R. A. Stone, and M. M. Civan Noninvasive Intraocular Pressure Measurements in Mice by Pneumotonometry Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3274 - 3280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Danias, K. C. Lee, M.-F. Zamora, B. Chen, F. Shen, T. Filippopoulos, Y. Su, D. Goldblum, S. M. Podos, and T. Mittag Quantitative Analysis of Retinal Ganglion Cell (RGC) Loss in Aging DBA/2NNia Glaucomatous Mice: Comparison with RGC Loss in Aging C57/BL6 Mice Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5151 - 5162. [Abstract] [Full Text]
|
|
|
|
|
|
M. Aihara, J. D. Lindsey, and R. N. Weinreb Aqueous Humor Dynamics in Mice Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5168 - 5173. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
