IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:1982-1992.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.02-0912
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishii, Y.
Right arrow Articles by Caprioli, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishii, Y.
Right arrow Articles by Caprioli, J.

Retinal Ganglion Cell Protection with Geranylgeranylacetone, a Heat Shock Protein Inducer, in a Rat Glaucoma Model

Yoko Ishii,1,2 Jacky M. K. Kwong,1 and Joseph Caprioli1

1From the Department of Ophthalmology, Jules Stein Eye Institute, University of California Los Angeles School of Medicine, Los Angeles, California; and the 2Department of Ophthalmology, University of Oita, Oita, Japan.

PURPOSE. To study the effects of geranylgeranylacetone (GGA) on the expression of inducible (HSP72) and constitutive (HSC70) heat shock proteins (HSPs) on retinal ganglion cells (RGCs) in a rat model of glaucoma.

METHODS. Adult Wistar rats were given intraperitoneal injections of GGA at 200 mg/kg daily. Western blot analysis and immunohistochemical staining for HSP72 and HSC70 were performed after 1, 3, and 7 days of treatment with GGA. After 7 days of GGA pretreatment, intraocular pressure (IOP) was elevated unilaterally by repeated trabecular argon laser photocoagulation 5 days after intracameral injection of india ink. After the first laser photocoagulation, GGA was administered twice a week. RGC survival was evaluated after 5 weeks of elevated IOP. Immunohistochemistry and TdT-mediated biotin-dUTP nick end labeling (TUNEL) were performed after 1 week of elevated IOP. Quercetin, an inhibitor of HSP expression, was also administered to a separate group.

RESULTS. There was increased expression of HSP72 in RGCs at 3 and 7 days after administration of GGA, but HSC70 was unchanged. After 5 weeks of elevated IOP, there was a 27% ± 6% loss of RGCs. The administration of GGA significantly reduced the loss of RGCs, lessened optic nerve damage, decreased the number of TUNEL-positive cells in the RGC layer, and increased HSP72. Quercetin abolished these protective effects.

CONCLUSIONS. These results demonstrate that systemic administration of GGA protects RGCs from glaucomatous damage in a rat model and suggest a novel pathway for neuroprotection in patients with glaucoma.




This article has been cited by other articles:


Home page
 IOVSHome page
Z. Yang, H. A. Quigley, M. E. Pease, Y. Yang, J. Qian, D. Valenta, and D. J. Zack
Changes in Gene Expression in Experimental Glaucoma and Optic Nerve Transection: The Equilibrium between Protective and Detrimental Mechanisms
Invest. Ophthalmol. Vis. Sci., December 1, 2007; 48(12): 5539 - 5548.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. M. Kim, K. H. Park, Y. J. Kim, H. J. Park, and D. M. Kim
Thermal Injury Induces Heat Shock Protein in the Optic Nerve Head In Vivo
Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 4888 - 4894.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E. M. Kniep, C. Roehlecke, N. Ozkucur, A. Steinberg, F. Reber, L. Knels, and R. H. W. Funk
Inhibition of Apoptosis and Reduction of Intracellular pH Decrease in Retinal Neural Cell Cultures by a Blocker of Carbonic Anhydrase.
Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 1185 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Katsuno, C. Sang, H. Adachi, M. Minamiyama, M. Waza, F. Tanaka, M. Doyu, and G. Sobue
Pharmacological induction of heat-shock proteins alleviates polyglutamine-mediated motor neuron disease
PNAS, November 15, 2005; 102(46): 16801 - 16806.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Tezel, X. Yang, and J. Cai
Proteomic Identification of Oxidatively Modified Retinal Proteins in a Chronic Pressure-Induced Rat Model of Glaucoma
Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3177 - 3187.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
M Seki, T Tanaka, H Matsuda, T Togano, K Hashimoto, J Ueda, T Fukuchi, and H Abe
Topically administered timolol and dorzolamide reduce intraocular pressure and protect retinal ganglion cells in a rat experimental glaucoma model
Br. J. Ophthalmol., April 1, 2005; 89(4): 504 - 507.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Tanito, Y.-W. Kwon, N. Kondo, J. Bai, H. Masutani, H. Nakamura, J. Fujii, A. Ohira, and J. Yodoi
Cytoprotective Effects of Geranylgeranylacetone against Retinal Photooxidative Damage
J. Neurosci., March 2, 2005; 25(9): 2396 - 2404.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology