IOVS Is this journal stale?
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:2192-2198.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.02-0410
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lambooij, A. C.
Right arrow Articles by Mooy, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lambooij, A. C.
Right arrow Articles by Mooy, C. M.

Insulin-like Growth Factor-I and its Receptor in Neovascular Age-Related Macular Degeneration

Antoinette C. Lambooij,1 Karel H. M. van Wely,2 Dicky J. Lindenbergh-Kortleve,3 Robert W. A. M. Kuijpers,1 Mike Kliffen,4 and Cornelia M. Mooy4,5

1From the Departments of Ophthalmology, 2Experimental Pathology, 3Pediatrics, and 4Pathology, Erasmus Medical Center Rotterdam, The Netherlands; and the 5Pathology Laboratory, Dordrecht, The Netherlands.

PURPOSE. The insulin-like growth factor (IGF)-I protein is a growth-promoting polypeptide that can act as an angiogenic agent in the eye. The purpose of the current study was to localize the expression of IGF-I and its receptor (IGF-IR) mRNA and IGF-IR protein in situ in the normal human eye and to examine the presence of expression in eyes with neovascular age-related macular degeneration (AMD).

METHODS. Formalin-fixed, paraffin-embedded slides of 4 normal control eyes and 14 eyes with choroidal neovascularization (CNV) secondary to AMD were examined. Three eyes with proliferative diabetic retinopathy were studied as the positive control. IGF-I and IGF-IR mRNA was detected by in situ hybridization with digoxigenin-labeled RNA probes. IGF-IR protein was studied by immunohistochemistry.

RESULTS. In the normal retina, IGF-I and IGF-IR mRNA expression was found throughout the neuroretinal layers, in the retinal pigment epithelium (RPE), and in some choriocapillary and retinal capillary endothelial cells. In eyes with CNV we found IGF and IGF-IR mRNA in capillary endothelial cells, some transdifferentiated RPE, and fibroblast-like cells. IGF-IR protein was found in normal eyes in all neuroretinal layers, in the RPE, and in the choroidal vessels. In eyes with CNV, IGF-IR protein was present in the RPE monolayer, in transdifferentiated RPE, and in newly formed vessels.

CONCLUSIONS. The colocalization of protein and receptor indicates an autocrine function of IGF-I in the normal human retina. Because IGF-I participates in ocular neovascularization, synthesis of IGF-IR and IGF-I in endothelial cells, RPE cells, and fibroblast-like cells in CNV may point toward a role for this growth factor in the pathogenesis of neovascular AMD.




This article has been cited by other articles:


Home page
 Br. J. Ophthalmol.Home page
A C Browning, H S Dua, and W M Amoaku
The effects of growth factors on the proliferation and in vitro angiogenesis of human macular inner choroidal endothelial cells
Br. J. Ophthalmol., July 1, 2008; 92(7): 1003 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. A. Economou, J. Wu, D. Vasilcanu, L. Rosengren, C. All-Ericsson, I. van der Ploeg, E. Menu, L. Girnita, M. Axelson, O. Larsson, et al.
Inhibition of VEGF Secretion and Experimental Choroidal Neovascularization by Picropodophyllin (PPP), an Inhibitor of the Insulin-like Growth Factor-1 Receptor
Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2620 - 2626.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
N. Nagai, Y. Oike, K. Izumi-Nagai, T. Urano, Y. Kubota, K. Noda, Y. Ozawa, M. Inoue, K. Tsubota, T. Suda, et al.
Angiotensin II Type 1 Receptor-Mediated Inflammation Is Required for Choroidal Neovascularization
Arterioscler. Thromb. Vasc. Biol., October 1, 2006; 26(10): 2252 - 2259.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. G. Slomiany and S. A. Rosenzweig
Hypoxia-Inducible Factor-1-Dependent and -Independent Regulation of Insulin-Like Growth Factor-1-Stimulated Vascular Endothelial Growth Factor Secretion
J. Pharmacol. Exp. Ther., August 1, 2006; 318(2): 666 - 675.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
O. Strauss
The Retinal Pigment Epithelium in Visual Function
Physiol Rev, July 1, 2005; 85(3): 845 - 881.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Morimoto, T. Miyoshi, S. Matsuda, Y. Tano, T. Fujikado, and Y. Fukuda
Transcorneal Electrical Stimulation Rescues Axotomized Retinal Ganglion Cells by Activating Endogenous Retinal IGF-1 System
Invest. Ophthalmol. Vis. Sci., June 1, 2005; 46(6): 2147 - 2155.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. G. Slomiany and S. A. Rosenzweig
IGF-1-Induced VEGF and IGFBP-3 Secretion Correlates with Increased HIF-1{alpha} Expression and Activity in Retinal Pigment Epithelial Cell Line D407
Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2838 - 2847.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology