HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, H. Articles by Zarbin, M. A. Search for Related Content PubMed PubMed Citation Articles by Wang, H. Articles by Zarbin, M. A.

Retinal Pigment Epithelium Wound Healing in Human Bruch’s Membrane Explants

¹From the University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark, New Jersey; and the ²Osaka University Medical School, Osaka, Japan.

PURPOSE. To compare retinal pigment epithelium (RPE) resurfacing on the RPE basement membrane and inner collagenous layer (ICL) in human submacular Bruch’s membrane explants.

METHODS. Debridements were created in RPE-choroid-sclera explants (mean donor age 71.91 ± 7.76 years) to create defects exposing the RPE basement membrane (RPEbm(+) defects), the ICL immediately below the RPE basement membrane (superficial ICL, [SICL]) or deeper layers of the ICL (DICL). Eleven pairs of eyes—four pairs with one eye having an RPEbm(+) defect and the fellow eye having an SICL defect and seven pairs with corresponding RPEbm(+) and DICL defects—were observed for 10 days by visualizing RPE ingrowth with 4',6'-diamino-2-phenylindole (DAPI) filters. At day 10, specimens were processed for scanning electron microscopy.

RESULTS. Resurfacing of localized RPE defects occurred to some degree in all 11 pairs of eyes. No significant difference in the percentage of resurfacing of RPEbm(+) defects (67.35% ± 18.82%) and SICL defects (64.26% ± 16.07%) was observed although healing of the SICL showed more variability in the morphology of RPE cells migrating into the defect. Significant differences in healing were observed between pairs with RPEbm(+) defects versus DICL defects (84.07% ± 15.35% and 54.00% ± 14.54% resurfacing, respectively). RPE ingrowth into DICL defects exhibited the greatest morphologic variability.

CONCLUSIONS. RPE basement membrane supports RPE resurfacing of localized RPE defects. The deeper portion of the ICL of aged submacular human Bruch’s membrane does not support RPE resurfacing to the same extent as does the RPE basement membrane. The poor RPE resurfacing observed in DICL defects mimics the histopathological findings in patients with age-related macular degeneration after excision of choroidal new vessels.

This article has been cited by other articles:

				A. G. Marneros, H. She, H. Zambarakji, H. Hashizume, E. J. Connolly, I. Kim, E. S. Gragoudas, J. W. Miller, and B. R. Olsen Endogenous endostatin inhibits choroidal neovascularization FASEB J, December 1, 2007; 21(14): 3809 - 3818. [Abstract] [Full Text] [PDF]

				J. F. Kiilgaard, J. U. Prause, M. Prause, E. Scherfig, M. H. Nissen, and M. la Cour Subretinal Posterior Pole Injury Induces Selective Proliferation of RPE Cells in the Periphery in In Vivo Studies in Pigs Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 355 - 360. [Abstract] [Full Text] [PDF]

				M A Zarbin Progressive RPE atrophy around disciform scars. Br. J. Ophthalmol., April 1, 2006; 90(4): 396 - 397. [Full Text] [PDF]

				S. Binder, I. Krebs, R.-D. Hilgers, A. Abri, U. Stolba, A. Assadoulina, L. Kellner, B. V. Stanzel, C. Jahn, and H. Feichtinger Outcome of Transplantation of Autologous Retinal Pigment Epithelium in Age-Related Macular Degeneration: A Prospective Trial Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 4151 - 4160. [Abstract] [Full Text] [PDF]