IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:2689-2693.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.02-1168
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ooto, S.
Right arrow Articles by Takahashi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ooto, S.
Right arrow Articles by Takahashi, M.

Induction of the Differentiation of Lentoids from Primate Embryonic Stem Cells

Sotaro Ooto,1,2 Masatoshi Haruta,2 Yoshihito Honda,1 Hiroshi Kawasaki,3,4 Yoshiki Sasai,3,5 and Masayo Takahashi2

1From the Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, and the 3Department of Neurobiology and Medical Embryology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan; the 2Department of Experimental Therapeutics, Translational Research Center, Kyoto University Hospital, Kyoto, Japan; the 4Department of Neurobiology, Duke University Medical Center, Durham, North Carolina; and the 5Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Japan.

PURPOSE. To produce lens cells from primate embryonic stem (ES) cells in a reproducible, controlled manner.

METHODS. Cynomologus monkey ES cells were induced to differentiate by stromal cell-derived inducing activity (SDIA). The lentoids produced by this treatment were processed for immunohistochemical and immunoblotting analysis. The effect of varying the concentration of fibroblast growth factor (FGF)-2 and the density of the ES colonies plated during the differentiation process were also examined.

RESULTS. After a 2- to 3-week induction period, lentoids were produced by a subpopulation of ES colonies. Western blot analysis and immunohistochemistry revealed that these lentoids expressed {alpha}A-crystallin and Pax6. The number of lentoids resulting from treatment increased with increasing FGF-2 concentration and plated colony density.

CONCLUSIONS. The differentiation of primate ES cells into lentoids can be achieved by treatment with SIDA. ES cells can be used to facilitate a greater understanding of the mechanisms functioning in differentiation in vivo and in vitro.




This article has been cited by other articles:


Home page
 Mol. Interv.Home page
P. A. Tsonis
Bridging Knowledge Gaps on the Long Road to Regeneration: Classical Models Meet Stem Cell Manipulation and Bioengineering
Mol. Interv., October 1, 2007; 7(5): 249 - 250.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
O. Medina-Martinez and M. Jamrich
Foxe view of lens development and disease
Development, April 15, 2007; 134(8): 1455 - 1463.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Ueno, M. Matsumura, K. Watanabe, T. Nakamura, F. Osakada, M. Takahashi, H. Kawasaki, S. Kinoshita, and Y. Sasai
Neural conversion of ES cells by an inductive activity on human amniotic membrane matrix
PNAS, June 20, 2006; 103(25): 9554 - 9559.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Ooto, T. Akagi, R. Kageyama, J. Akita, M. Mandai, Y. Honda, and M. Takahashi
Potential for neural regeneration after neurotoxic injury in the adult mammalian retina
PNAS, September 14, 2004; 101(37): 13654 - 13659.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Tabata, Y. Ouchi, H. Kamiya, T. Manabe, K.-i. Arai, and S. Watanabe
Specification of the Retinal Fate of Mouse Embryonic Stem Cells by Ectopic Expression of Rx/rax, a Homeobox Gene
Mol. Cell. Biol., May 15, 2004; 24(10): 4513 - 4521.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Haruta, Y. Sasai, H. Kawasaki, K. Amemiya, S. Ooto, M. Kitada, H. Suemori, N. Nakatsuji, C. Ide, Y. Honda, et al.
In Vitro and In Vivo Characterization of Pigment Epithelial Cells Differentiated from Primate Embryonic Stem Cells
Invest. Ophthalmol. Vis. Sci., March 1, 2004; 45(3): 1020 - 1025.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology