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(Investigative Ophthalmology and Visual Science. 2003;44:2694-2701.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.02-0822
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Effects of Astaxanthin on Lipopolysaccharide-Induced Inflammation In Vitro and In Vivo

Kazuhiro Ohgami,1 Kenji Shiratori,1 Satoshi Kotake,1 Tomomi Nishida,2 Nobuhisa Mizuki,2 Kazunaga Yazawa,3 and Shigeaki Ohno1

1From the Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan; the 2Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan; and the 3Laboratory of Nutraceuticals and Functional Foods Science, Graduate School of Fisheries Science, Tokyo University of Fisheries, Tokyo, Japan.

PURPOSE. Astaxanthin (AST) is a carotenoid that is found in marine animals and vegetables. Several previous studies have demonstrated that AST exhibits a wide variety of biological activities including antioxidant, antitumor, and anti-Helicobacter pylori effects. In this study, attention was focused on the antioxidant effect of AST. The object of the present study was to investigate the efficacy of AST in endotoxin-induced uveitis (EIU) in rats. In addition, the effect of AST on endotoxin-induced nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor (TNF)-{alpha} production in a mouse macrophage cell line (RAW 264.7) was studied in vitro.

METHODS. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). AST or prednisolone was administered intravenously at 30 minutes before, at the same time as, or at 30 minutes after LPS treatment. The number of infiltrating cells and protein concentration in the aqueous humor collected at 24 hours after LPS treatment was determined. RAW 264.7 cells were pretreated with various concentrations of AST for 24 hours and subsequently stimulated with 10 µg/mL of LPS for 24 hours. The levels of PGE2, TNF-{alpha}, and NO production were determined in vivo and in vitro.

RESULTS. AST suppressed the development of EIU in a dose-dependent fashion. The anti-inflammatory effect of 100 mg/kg AST was as strong as that of 10 mg/kg prednisolone. AST also decreased production of NO, activity of inducible nitric oxide synthase (NOS), and production of PGE2 and TNF-{alpha} in RAW264.7 cells in vitro in a dose-dependent manner.

CONCLUSIONS. This study suggests that AST has a dose-dependent ocular anti-inflammatory effect, by the suppression of NO, PGE2, and TNF-{alpha} production, through directly blocking NOS enzyme activity.




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