IOVS Proceedings of the National Academy of Sciences
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(Investigative Ophthalmology and Visual Science. 2003;44:4006-4011.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0040
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Interleukin-6 in Retinal Ischemia Reperfusion Injury in Rats

Ruben N. Sanchez,1 Candy K. Chan,1 Sumit Garg,1 Jacky M. K. Kwong,2 Micheline J. Wong,1 Alfredo A. Sadun,1 and Tim T. Lam1

1From the Department of Ophthalmology, Doheny Eye Institute, University of Southern California, Keck School of Medicine, Los Angeles, California; and the 2Glaucoma Division, Department of Ophthalmology, Jules Stein Eye Institute, University of California at Los Angeles School of Medicine, Los Angeles, California.

PURPOSE. To study the role of interleukin (IL)-6 after retinal ischemia–reperfusion (I/R) injury in rats.

METHODS. Intraocular pressure of adult male Lewis albino rats was raised to create retinal ischemia for 1 hour. Retinal reperfusion was reestablished, and the animals were killed at various time points after the injury. Their eyes were enucleated and processed for immunohistochemistry to detect IL-6 and ED-1 (a marker of microglial/phagocytic cells), enzyme-linked immunosorbent assay (ELISA) of IL-6 protein, and semiquantitative real-time RT-PCR for IL-6 mRNA. The neuroprotective effect of IL-6 was evaluated by giving intravitreal injections of 150 or 300 ng rat recombinant IL-6 to eyes immediately after I/R injury and counting cresyl violet–stained retinal ganglion cell layer cells (RGCLCs) and fluorochrome-labeled retinal ganglion cells (RGCs) on flat preparations of retinas at 7 days.

RESULTS. IL-6–positive cells appeared after I/R injury in the inner plexiform layer (IPL) and the inner nuclear layer (INL). Their numbers were significantly higher 18 hours after the injury, and most of these cells were also ED-1 positive. ELISA showed noticeable increases in endogenous retinal IL-6 protein levels 8 hours after I/R injury. Semiquantitative real-time RT-PCR showed significant increases in endogenous retinal IL-6 mRNA levels between 2 and 18 hours. Exogenously added IL-6 prevented between 50% and 70% of RGC loss after I/R injury.

CONCLUSIONS. IL-6 is upregulated after retinal I/R injury, and its expression by microglia/phagocytic cells may protect RGC layer neurons from I/R injury. Exogenously added IL-6 protects the inner retina after I/R injury.




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