IOVS Journal of General Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:4044-4053.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.02-1175
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Del Priore, L. V.
Right arrow Articles by Kaplan, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Del Priore, L. V.
Right arrow Articles by Kaplan, H. J.

Triple Immune Suppression Increases Short-Term Survival of Porcine Fetal Retinal Pigment Epithelium Xenografts

Lucian V. Del Priore,1 Osamu Ishida,1 Eric W. Johnson,2 Yaohua Sheng,1 Douglas B. Jacoby,2 Lee Geng,1 Tongalp H. Tezel,1,3 and Henry J. Kaplan3

1From the Department of Ophthalmology, Harkness Eye Institute, Columbia University, New York, New York; 2Diacrin Inc, Charlestown, MA; and the 3Department of Ophthalmology and Visual Sciences, Lions Eye Institute, University of Louisville, Louisville, Kentucky.

PURPOSE. To determine the effect of triple drug immune suppression on RPE xenograft survival in the fetal pig after transplantation into the albino rabbit subretinal space.

METHODS. Primary RPE microaggregates (approximately 40,000 RPE cells) were injected into the subretinal space of 24 albino rabbits, with half the rabbits maintained on triple systemic immune suppression. RPE survival was estimated with a DNA probe (porcine DNA repeat element; PRE) against a porcine-specific repetitive chromosomal marker or a RAM-11 antibody against rabbit macrophages.

RESULTS. Numerous pigmented cells were visible in the subretinal space at all time points, but most pigment-containing cells 4 weeks or more after surgery were RAM-11 positive and PRE negative. The number of PRE-positive cells in the immune-suppressed group (4193 ± 2461, 1184 ± 1502, and 541 ± 324 at 4, 8, and 12 weeks, respectively) was greater than in immune-competent control animals (292 ± 506, 193 ± 173, and 111 ± 96), but the difference was only statistically significant at 4 weeks. The time-dependent decrease in PRE-positive cells was more pronounced in immune-suppressed animals. Image analysis performed on serial fundus photographs and fluorescein angiograms did not detect any difference in the appearance of the grafts in immune-suppressed versus immune-competent animals.

CONCLUSIONS. Systemic immune suppression increased the 4-week survival of porcine RPE xenografts in the albino rabbit subretinal space, but there was poor survival in immune-suppressed and -competent animals 12 weeks after surgery. Many pigment-containing cells 4 or more weeks after surgery were PRE negative, indicating that they are of host origin.




This article has been cited by other articles:


Home page
 IOVSHome page
P. J. Francis, S. Wang, Y. Zhang, A. Brown, T. Hwang, T. J. McFarland, B. G. Jeffrey, B. Lu, L. Wright, B. Appukuttan, et al.
Subretinal Transplantation of Forebrain Progenitor Cells in Nonhuman Primates: Survival and Intact Retinal Function
Invest. Ophthalmol. Vis. Sci., July 1, 2009; 50(7): 3425 - 3431.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Wang, B. Lu, S. Girman, T. Holmes, N. Bischoff, and R. D. Lund
Morphological and Functional Rescue in RCS Rats after RPE Cell Line Transplantation at a Later Stage of Degeneration
Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 416 - 421.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
H. Cai, M. C. Shin, T. H. Tezel, H. J. Kaplan, and L. V. Del Priore
Use of iris pigment epithelium to replace retinal pigment epithelium in age-related macular degeneration: a gene expression analysis.
Arch Ophthalmol, September 1, 2006; 124(9): 1276 - 1285.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Yoshioka, T. Abe, R. Wakusawa, T. Moriya, S. Mochizuki, Y. Saigo, T. Saito, H. Murata, Y. Tokita, T. Iseya, et al.
Recombinant AAV-Transduced Iris Pigment Epithelial Cell Transplantation May Transfer Vector to Native RPE but Suppress Systemic Dissemination
Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 745 - 752.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Wang, B. Lu, P. Wood, and R. D. Lund
Grafting of ARPE-19 and Schwann Cells to the Subretinal Space in RCS Rats
Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2552 - 2560.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
L. V. Del Priore, T. H. Tezel, and H. J. Kaplan
Survival of Allogeneic Porcine Retinal Pigment Epithelial Sheets after Subretinal Transplantation
Invest. Ophthalmol. Vis. Sci., March 1, 2004; 45(3): 985 - 992.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
From the Library
Br. J. Ophthalmol., November 1, 2003; 87(11): 1436 - 1436.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology