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(Investigative Ophthalmology and Visual Science. 2004;45:110-113.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0046
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Proliferative Effects of Heparin-Binding Epidermal Growth Factor–like Growth Factor on Pterygium Epithelial Cells and Fibroblasts

Timothy M. Nolan,1 Nick Di Girolamo,1 Minas T. Coroneo,2 and Denis Wakefield1

1From the Inflammation Research Unit, School of Medical Sciences, University of New South Wales, Sydney, Australia; and the 2Department of Ophthalmology, Prince of Wales Hospital, Randwick, New South Wales, Australia.

PURPOSE. To investigate the growth promoting and chemotactic effects of heparin binding epidermal growth factor-like growth factor (HB-EGF), recently shown to be upregulated by ultraviolet irradiation in pterygium-derived epithelium cells (PECs) and pterygium fibroblasts (PFs).

METHODS. PECs and PFs were incubated with various concentrations of HB-EGF. Cell proliferation was evaluated by measurement of [3H]thymidine incorporation. The potential chemotactic effect of HB-EGF on these two cell lines was assessed with migration assays, using modified Boyden chambers and checkerboard analysis.

RESULTS. Incubation of PECs and PFs with HB-EGF resulted in a significant increase in [3H]thymidine incorporation compared with that of control cells. HB-EGF stimulated chemotaxis of both PECs and PFs. Maximum stimulation occurred at 1 ng/mL for PFs and 10 ng/mL for PECs. These effects were abolished by the addition of a neutralizing antibody to HB-EGF.

CONCLUSIONS. The findings demonstrate the potential proliferative and chemotactic effects of HB-EGF on both PECs and PFs. This is the first study to illustrate the positive effect of a specific growth or chemotactic factor on the cellular elements of a pterygium.




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 Am. J. Pathol.Home page
N. Di Girolamo, M. Coroneo, and D. Wakefield
Epidermal Growth Factor Receptor Signaling Is Partially Responsible for the Increased Matrix Metalloproteinase-1 Expression in Ocular Epithelial Cells after UVB Radiation
Am. J. Pathol., August 1, 2005; 167(2): 489 - 503.
[Abstract] [Full Text] [PDF]


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