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(Investigative Ophthalmology and Visual Science. 2004;45:3951-3954.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.04-0381
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Porcine Corneal Epithelial Cells Consist of High- and Low-Integrin ß1–Expressing Populations

Ken-ichi Endo, Takahiro Nakamura, Satoshi Kawasaki, and Shigeru Kinoshita

From the Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.

PURPOSE. Immunohistochemically, all cells in the porcine corneal epithelium, from the superficial to the basal layer, express integrin ß1. Flow cytometric study has shown that they comprise integrin ß1 high-expressing (ß12+) and low-expressing (ß1+) populations. This study was undertaken to determine their proliferation characteristics.

METHODS. Epithelial cells from porcine corneas were sorted and labeled with anti-integrin ß1 antibody and a fluorescent-dye–conjugated secondary antibody. The fluorescent intensity of labeled cells was analyzed and ß12+ and ß1+ cells were cultured in an adhesive-coated culture plate.

RESULTS. Flow cytometry demonstrated that the epithelial cells comprised two distinct populations with a similar ratio throughout the cornea. Whereas ß12+ cells attached and grew to confluence in the plate, ß1+ attached only transiently to the plate and exhibited minimal growth.

CONCLUSIONS. The data indicate that the porcine cornea contains two distinct populations of epithelial cells, one exhibiting high and the other low integrin ß1 expression. The observation that ß12+ cells had greater growth potential suggests that they may represent an enriched population of transit-amplifying cells.






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