IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2004;45:3951-3954.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.04-0381
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Endo, K.-i.
Right arrow Articles by Kinoshita, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Endo, K.-i.
Right arrow Articles by Kinoshita, S.

Porcine Corneal Epithelial Cells Consist of High- and Low-Integrin ß1–Expressing Populations

Ken-ichi Endo, Takahiro Nakamura, Satoshi Kawasaki, and Shigeru Kinoshita

From the Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.

PURPOSE. Immunohistochemically, all cells in the porcine corneal epithelium, from the superficial to the basal layer, express integrin ß1. Flow cytometric study has shown that they comprise integrin ß1 high-expressing (ß12+) and low-expressing (ß1+) populations. This study was undertaken to determine their proliferation characteristics.

METHODS. Epithelial cells from porcine corneas were sorted and labeled with anti-integrin ß1 antibody and a fluorescent-dye–conjugated secondary antibody. The fluorescent intensity of labeled cells was analyzed and ß12+ and ß1+ cells were cultured in an adhesive-coated culture plate.

RESULTS. Flow cytometry demonstrated that the epithelial cells comprised two distinct populations with a similar ratio throughout the cornea. Whereas ß12+ cells attached and grew to confluence in the plate, ß1+ attached only transiently to the plate and exhibited minimal growth.

CONCLUSIONS. The data indicate that the porcine cornea contains two distinct populations of epithelial cells, one exhibiting high and the other low integrin ß1 expression. The observation that ß12+ cells had greater growth potential suggests that they may represent an enriched population of transit-amplifying cells.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Association for Research in Vision and Ophthalmology