IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2004;45:641-647.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0930
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gandorfer, A.
Right arrow Articles by Charteris, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gandorfer, A.
Right arrow Articles by Charteris, D.

Posterior Vitreous Detachment Induced by Microplasmin

Arnd Gandorfer,1,2,3 Matthias Rohleder,1,2,3 Charanjit Sethi,2 Dominik Eckle,1 Ulrich Welge-Lüssen,1 Anselm Kampik,1 Philip Luthert,3 and David Charteris2

1From the Department of Ophthalmology, University Eye Hospital, Ludwig-Maximilians-University, Munich, Germany; the 2Vitreoretinal Research Unit, Moorfields Eye Hospital, United Kingdom; the 3Department of Pathology, Institute of Ophthalmology, London, United Kingdom.

PURPOSE. To demonstrate the efficacy of microplasmin in inducing posterior vitreous detachment (PVD) and to evaluate the human and the feline retina after treatment.

METHODS. Thirteen human donor eyes were injected with 62.5, 125, or 188 µg microplasmin. The 13 fellow eyes received balanced salt solution. Four of the microplasmin-treated eyes received an additional intravitreal gas injection. After incubation at 37°C for 30 minutes, all globes were placed in 4% paraformaldehyde. Retinal specimens were processed for scanning (SEM) and transmission (TEM) electron microscopy. Five feline eyes were injected with 14.5- or 25-µg microplasmin. Animals were killed after 1 day, 3 days, or 3 weeks, and retinal specimens were evaluated by electron and confocal microscopy.

RESULTS. In all control eyes, SEM demonstrated the cortical vitreous covering the inner limiting membrane (ILM). Intravitreal injection of 125 or 188 µg microplasmin resulted in complete PVD. After treatment with 62.5 µg microplasmin, SEM revealed collagen fibrils covering the ILM. Additional gas injection did not change the dose necessary for PVD. In vivo in cats, 25 µg microplasmin resulted in complete PVD after 3 days. After 3 weeks, there was complete PVD with both doses of microplasmin. The retina and the ILM were well preserved in all eyes.

CONCLUSIONS. Both after death and in vivo, microplasmin induces a dose-dependent cleavage between the vitreous cortex and the ILM without morphologic alterations of the retina. In the feline eye, there is no cellular response of retinal glial cells or neurons.




This article has been cited by other articles:


Home page
 IOVSHome page
T. Sakuma, M. Tanaka, A. Mizota, J. Inoue, and S. Pakola
Safety of In Vivo Pharmacologic Vitreolysis with Recombinant Microplasmin in Rabbit Eyes
Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3295 - 3299.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
F. Wang, Z. Wang, X. Sun, F. Wang, X. Xu, and X. Zhang
Safety and Efficacy of Dispase and Plasmin in Pharmacologic Vitreolysis
Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 3286 - 3290.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Association for Research in Vision and Ophthalmology