IOVS Journal of Nutrition
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2004;45:1041-1046.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-1017
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bishop, P. N.
Right arrow Articles by Bos, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bishop, P. N.
Right arrow Articles by Bos, K. J.

Age-Related Changes on the Surface of Vitreous Collagen Fibrils

Paul N. Bishop,1,2 David F. Holmes,1 Karl E. Kadler,1 David McLeod,2 and Kees Jan Bos1,2

1From the Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences and 2Research Group in Eye & Vision Science, School of Medicine, University of Manchester, Manchester, United Kingdom.

PURPOSE. To determine whether aging vitreous collagen fibrils undergo ultrastructural changes that might underlie vitreous liquefaction and posterior vitreous detachment.

METHODS. Vitreous collagen fibrils from 21 human subjects (age range, 3–89 years) and from bovine eyes were isolated on electron microscopy grids. Cupromeronic blue labeling in the presence of 0.3 M MgCl2 and immunogold labeling for collagen types II and IX were analyzed by transmission electron microscopy.

RESULTS. Aging was associated with marked changes on the surface of human vitreous collagen fibrils, including an exponential loss of type IX collagen along with its chondroitin sulfate side-chains (half-life, 11 years) and a fourfold increase in the exposure of type II collagen.

CONCLUSIONS. Despite being a minor component of vitreous collagen fibrils, type IX collagen, probably by virtue of its chondroitin sulfate side-chains, shields type II collagen from exposure on the fibril surface. With aging, this shielding diminishes, resulting in the surface exposure of "sticky" type II collagen and thus predisposing the vitreous collagen fibrils to fusion. These changes could underlie vitreous liquefaction and weakening of vitreoretinal adhesion.




This article has been cited by other articles:


Home page
 IOVSHome page
T. L. Ponsioen, M. v. Deemter, R. A. Bank, J. M. Snabel, G. S. Zijlstra, R. J. van der Worp, J. M. M. Hooymans, and L. I. Los
Mature Enzymatic Collagen Cross-Links, Hydroxylysylpyridinoline and Lysylpyridinoline, in the Aging Human Vitreous
Invest. Ophthalmol. Vis. Sci., March 1, 2009; 50(3): 1041 - 1046.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. F. Holmes and K. E. Kadler
The 10+4 microfibril structure of thin cartilage fibrils
PNAS, November 14, 2006; 103(46): 17249 - 17254.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Halfter, U. Winzen, P. N. Bishop, and A. Eller
Regulation of eye size by the retinal basement membrane and vitreous body.
Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3586 - 3594.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
V. J. Hindson, J. T. Gallagher, W. Halfter, and P. N. Bishop
Opticin Binds to Heparan and Chondroitin Sulfate Proteoglycans
Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4417 - 4423.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Halfter, S. Dong, B. Schurer, C. Ring, G. J. Cole, and A. Eller
Embryonic Synthesis of the Inner Limiting Membrane and Vitreous Body
Invest. Ophthalmol. Vis. Sci., June 1, 2005; 46(6): 2202 - 2209.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Association for Research in Vision and Ophthalmology