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1From the Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, United Kingdom; the 2Division of Reproductive and Developmental Sciences, Genes and Development Group, University of Edinburgh, Edinburgh, Scotland, United Kingdom; and the 3Comparative and Developmental Genetics Section, Medical Research Council [MRC] Human Genetics Unit, Edinburgh, Scotland, United Kingdom.
PURPOSE. To investigate the etiology of corneal dysfunction in the Pax6+/- mouse model of aniridia-related keratopathy.
METHODS. Mosaic patterns of X-gal staining were compared in the corneal and limbal epithelia of female Pax6+/- and Pax6+/+ littermates, age 3 to 28 weeks, hemizygous for an X-linked LacZ transgene, and Pax6+/+, LacZ-
Pax6+/+, LacZ+ and Pax6+/+, LacZ-Pax6+/-, LacZ+ chimeras. Histologic examination of chimeric corneas was performed.
RESULTS. Disrupted patterns of X-gal staining showed that heterozygosity for Pax6 perturbed clonal patterns of growth and development in the corneal and limbal epithelium. Centripetal migration of Pax6+/- corneal epithelial cells was diverted. Normal patterns of centripetal Pax6+/- cell migration and epithelial morphology were restored in Pax6+/+Pax6+/- chimeras. Fewer, larger clones of limbal stem cells were present in Pax6+/- eyes, compared with wild-type. In the chimeras, Pax6+/- limbal stem cells were cell-autonomously depleted or less efficient than wild-type cells at producing progeny to populate the corneal epithelium.
CONCLUSIONS. The correct Pax6 dosage is necessary for normal clonal growth during corneal development, normal limbal stem cell activity, and correct corneal epithelial cell migration. Disruption of normal cell movement in heterozygotes may be the consequence of failure of nonautonomous guidance cues. Degeneration of the corneal surface in aniridia-related keratopathy relates to both a deficiency within the limbal stem cell niche and nonautonomous diversion of corneal epithelial cell migration.
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