IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2004;45:1688-1691.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0568
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yen, M.-Y.
Right arrow Articles by Wei, Y.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yen, M.-Y.
Right arrow Articles by Wei, Y.-H.

Increased 8-Hydroxy-2'-Deoxyguanosine in Leukocyte DNA in Leber’s Hereditary Optic Neuropathy

May-Yung Yen,1 Shu-Huei Kao,2 An-Guor Wang,1 and Yau-Huei Wei3,4

1From the Department of Ophthalmology, Taipei Veterans General Hospital, and the 3Department of Biochemistry and the 4Center for Cellular and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, Republic of China; and the 2Graduate Institute of Biomedical Technology, Taipei Medical University, Taipai, Taiwan, Republic of China.

PURPOSE. This study was conducted to test the hypothesis that oxidative stress is involved in the pathogenesis of Leber’s hereditary optic neuropathy (LHON). The level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidized DNA base common in cells undergoing oxidative stress, was measured in leukocyte DNA from patients with LHON and normal control subjects.

METHODS. The 8-OHdG and deoxyguanosine (dG) content in leukocyte DNA from 25 patients with LHON with an 11778 mitochondrial (mt)DNA mutation, 14 asymptomatic maternal relatives, and 27 unrelated normal control subjects were measured using high-performance liquid chromatography and electrochemical detection methods.

RESULTS. The mean 8-OHdG/105 dG ratio from leukocyte DNA was 1.34 ± 0.99 in patients with LHON, 1.00 ± 0.91 in their asymptomatic maternal relatives, and 0.31 ± 0.20 in normal control subjects, respectively. There was a statistically significant difference in the mean 8-OHdG/105 dG ratio between patients with LHON and normal control subjects and between asymptomatic maternal relatives and normal control subjects. The difference between patients with LHON and asymptomatic maternal relatives did not reach statistical significance.

CONCLUSIONS. Patients with LHON with an 11778 mtDNA mutation had higher oxidative DNA damage. Oxidative stress has a key role in the pathogenesis of LHON.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Association for Research in Vision and Ophthalmology