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Insertion of a Pax6 Consensus Binding Site into the A-Crystallin Promoter Acts as a Lens Epithelial Cell Enhancer in Transgenic Mice

¹From the Center for Molecular and Human Genetics, Columbus Children’s Research Institute, Columbus, Ohio; the ²Graduate Program in Molecular, Cellular, and Developmental Biology, College of Biological Sciences, The Ohio State University, Columbus, Ohio; the ³Department of Cellular and Molecular Biology, Baylor College of Medicine, Houston, Texas; and the ⁴Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, Ohio.

PURPOSE. Although the murine A-crystallin promoter is the most commonly used promoter for achieving transgene expression in the developing lens, this promoter directs transgene expression efficiently only in lens fiber cells. The purpose of the present study was to generate promoters capable of directing transgene expression to the entire lens but not to the corneal epithelium.

METHODS. Transgenic mice were generated with fragments of the murine A- and B-crystallin promoters, as well as with an A-crystallin promoter engineered with the insertion of a Pax6 consensus binding site driving either human growth hormone (hGH) or Cre recombinase genes. hGH expression was evaluated by in situ hybridization and immunohistochemistry. Cre expression was revealed by x-gal staining after crossing Cre transgenic mice with a Cre reporter strain.

RESULTS. Within the lens, the –214/+38 B-crystallin promoter fragment directed transgene expression in the lens epithelium, but not in fiber cells. The native –282/+43 A-crystallin promoter drove transgene expression in the lens fiber cells of several independent lines of transgenic mice, but none of these mice demonstrated significant transgene expression in the lens epithelium. In contrast, the insertion of a 32-bp sequence containing a Pax6 consensus binding site into the –282/+43 A-crystallin promoter reproducibly led to transgene expression in the lens epithelium as well as the lens fiber cells.

CONCLUSIONS. The inclusion of a Pax6 consensus binding site within the –282/+43 A-crystallin promoter enhances the ability of this promoter to drive transgene expression in the lens epithelium.

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