IOVS Applied and Environmental Microbiology
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(Investigative Ophthalmology and Visual Science. 2004;45:2115-2121.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0929
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Peroxynitrite Reaction with Eye Lens Proteins: {alpha}-Crystallin Retains Its Activity Despite Modification

Geetha Thiagarajan, Jaganathan Lakshmanan, Madhavilatha Chalasani, and Dorairajan Balasubramanian

From the Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L. V. Prasad Eye Institute, Hyderabad, India.

PURPOSE. Peroxynitrite is a highly potent reactive oxygen/nitrogen species present in the environment and also endogenously in the eye, that causes a variety of disorders. This study was undertaken to look at the oxidative damage that peroxynitrite causes to the proteins of the lens and the functional consequences thereof.

METHODS. Peroxynitrite was allowed to react with {alpha}-, ß-, and {gamma}-crystallins. The formation of nitrotyrosine and nitrotryptophan, dityrosine, protein covalent cross-links, and chain degradation products were monitored by spectroscopy and SDS-PAGE. Conformational changes occurring in the protein were monitored with circular dichroism spectroscopy. The chaperoning ability of {alpha}-crystallin was assayed by monitoring its ability to inhibit the self-aggregation of two test proteins: ß-crystallin and insulin.

RESULTS. Peroxynitrite reaction produced nitrotyrosine, nitrotryptophan, and dityrosine, nondisulfide covalent cross-linked aggregates, and peptide chain degradation. The hydroxyl radicals produced by peroxynitrite caused more chain degradation than did the carbonate radicals. The oxidative reaction caused increased conformational disorder. The yield was highest in {gamma}-crystallin and least in {alpha}-crystallin. The chaperoning ability of {alpha}-crystallin was not affected.

CONCLUSIONS. Peroxynitrite reacts with lens proteins, causing extensive covalent chemical changes. However, {alpha}-crystallin retains its chaperoning ability, despite the oxidative changes caused by the peroxynitrite reaction, indicating its functional robustness.




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