IOVS Arteriosclerosis, Thrombosis, and Vascular Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2005;46:3502-3506.)
© 2005 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.05-0309
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ozerdem, U.
Right arrow Articles by Li, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ozerdem, U.
Right arrow Articles by Li, A.

Contribution of Bone Marrow–Derived Pericyte Precursor Cells to Corneal Vasculogenesis

Ugur Ozerdem,1 Kari Alitalo,2 Petri Salven,3 and Andrew Li4

1From the La Jolla Institute for Molecular Medicine, La Jolla, California; 2Biomedicum Helsinki, 3University of Helsinki, Helsinki, Finland; 4University of California San Diego, La Jolla, California.

PURPOSE. Bone-marrow (BM)–derived hematopoietic precursor cells are thought to participate in the growth of blood vessels during postnatal vasculogenesis. In this investigation, multichannel laser scanning confocal microscopy and quantitative image analysis were used to study the fate of BM-derived hematopoietic precursor cells in corneal neovascularization.

METHODS. A BM-reconstituted mouse model was used in which the BM from enhanced green fluorescent protein (GFP)–positive mice was transplanted into C57BL/6 mice. Basic fibroblast growth factor (bFGF) was used to induce corneal neovascularization in mice. The vasculogenic potential of adult BM-derived cells and their progeny were tested in this in vivo model. Seventy-two histologic sections selected by systematic random sampling from four mice were immunostained and imaged with a confocal microscope and analyzed with image-analysis software.

RESULTS. BM-derived endothelial cells did not contribute to bFGF-induced neovascularization in the cornea. BM-derived periendothelial vascular mural cells (pericytes) were detected at sites of neovascularization, whereas endothelial cells of blood vessels originated from preexisting blood vessels in limbal capillaries. Fifty three percent of all neovascular pericytes originated from BM, and 47% of them originated from preexisting corneoscleral limbus capillaries. Ninety-six percent and 92% of BM-derived pericytes also expressed CD45 and CD11b, respectively, suggesting their hematopoietic origin from the BM.

CONCLUSIONS. Pericytes of new corneal vessels have a dual source: BM and preexisting limbal capillaries. These findings establish BM as a significant effector organ in corneal disorders associated with neovascularization.




This article has been cited by other articles:


Home page
 BloodHome page
A. K. Horst, T. Bickert, N. Brewig, P. Ludewig, N. van Rooijen, U. Schumacher, N. Beauchemin, W. D. Ito, B. Fleischer, C. Wagener, et al.
CEACAM1+ myeloid cells control angiogenesis in inflammation
Blood, June 25, 2009; 113(26): 6726 - 6736.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
M. R. Hayden, P. R. Karuparthi, J. Habibi, G. Lastra, K. Patel, C. Wasekar, C. M. Manrique, U. Ozerdem, S. Stas, and J. R. Sowers
Ultrastructure of Islet Microcirculation, Pericytes and the Islet Exocrine Interface in the HIP Rat Model of Diabetes
Experimental Biology and Medicine, September 1, 2008; 233(9): 1109 - 1123.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
U. Tigges, E. G. Hyer, J. Scharf, and W. B. Stallcup
FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages
Development, February 1, 2008; 135(3): 523 - 532.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
T. Hisatomi, K.-h. Sonoda, F. Ishikawa, H. Qiao, T. Nakazawa, M. Fukata, T. Nakamura, K. Noda, S. Miyahara, M. Harada, et al.
Identification of resident and inflammatory bone marrow derived cells in the sclera by bone marrow and haematopoietic stem cell transplantation
Br. J. Ophthalmol., April 1, 2007; 91(4): 520 - 526.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
C. Lamagna and G. Bergers
The bone marrow constitutes a reservoir of pericyte progenitors
J. Leukoc. Biol., October 1, 2006; 80(4): 677 - 681.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Association for Research in Vision and Ophthalmology