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Effect of TGF-ß2 and Anti–TGF-ß2 Antibody in a New In Vivo Rodent Model of Posterior Capsule Opacification

¹From the Department of Ophthalmology and the ²Bone Research Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, United Kingdom.

PURPOSE. This study evaluated the effect of transforming growth factor (TGF)-ß2 and anti–TGF-ß2 antibody in a rodent model of posterior capsule opacification (PCO).

METHODS. An extracapsular lens extraction (ECLE) was performed in 72 Sprague–Dawley rats. At the end of the procedure, 10 µL TGF-ß2 (TGF-ß2–treated group), fetal calf serum (FCS)/phosphate-buffered saline (PBS; FCS/PBS-treated control group), a human monoclonal TGF-ß2 antibody (anti–TGF-ß2–treated group), or a null control IgG4 antibody (null antibody–treated control group) was injected into the capsule. Animals were killed 3 and 14 days postoperatively. Eyes were evaluated clinically prior to euthanatization, then enucleated and processed for light microscopy and immunohistochemistry afterward. PCO was evaluated clinically and histopathologically. Student’s t-test and ² were used to assess differences between groups.

RESULTS. There were no statistically significant clinical or histopathological differences in degree of PCO between the TGF-ß2– and FCS/PBS-treated groups at 3 and 14 days after ECLE. Nor were there differences between the anti–TGF-ß2– and the null antibody–treated groups, with the exception of the histopathology score for capsule wrinkling 3 days after ECLE (P = 0.02). -Smooth-muscle actin staining was observed in the lens capsular bag only in areas where there was close contact with the iris.

CONCLUSIONS. No sustained effect of TGF-ß2 or anti–TGF-ß2 antibody on PCO was found in rodents at the dose and timing administered in this study. Iris cells may play a role in the process of epithelial mesenchymal transition linked to PCO.

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