| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1From the Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan; and the 2Departments of Medicine and Biosystemic Science and 3Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Kyushu, Japan.
PURPOSE. Bone marrow (BM)–derived stem cells are thought to possess extensive differentiation capacity. The present study was conducted to investigate the characteristics and distribution of these cells in the normal mouse cornea.
METHODS. BM cells and BM-derived hematopoietic stem/progenitor cells (HSCs) from enhanced GFP (eGFP) transgenic mice (lin–, Sca-1+) were intravenously transplanted into irradiated wild-type C57BL/6 mice. At 4 to 6 months after transplantation, the mice were killed, and their whole corneas examined by histologic and immunohistochemical methods (CD11c, CD11b, and CD45).
RESULTS. At 2 weeks after BM cell transplantation, GFP+ cells gradually migrated into the cornea from the limbal area. At 2 to 6 months, they were distributed over the entire cornea. In cross sections of whole cornea, GFP+ cells comprised 27.3% ± 11.1% (BM) and 24.0% ± 8.01% (HSC) of total cells in the peripheral corneal stroma. In the center of the corneal stroma, GFP+ cells were 7.58% ± 2.63% (BM) and 8.06% ± 1.76% (HSC) of total cells. Immunohistochemistry showed that GFP+ CD11c+, CD11b+, CD11c–, and CD11b– cells occupied the entire corneal stroma.
CONCLUSIONS. The present study provides direct evidence of the distribution of BM-derived cells in the mouse cornea. Immunohistochemical study showed that some of these cells are BM-derived antigen-presenting cells such as dendritic cells and macrophages. Some elements of BM-derived cells may continue to exist in the corneal stroma.
This article has been cited by other articles:
|
|
 |
|
  K. Gentil and E. Pearlman Gamma Interferon and Interleukin-1 Receptor 1 Regulate Neutrophil Recruitment to the Corneal Stroma in a Murine Model of Onchocerca volvulus Keratitis Infect. Immun., April 1, 2009; 77(4): 1606 - 1612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kaneko, K. M. Nishiguchi, M. Nakamura, S. Kachi, and H. Terasaki Characteristics of Bone Marrow-Derived Microglia in the Normal and Injured Retina Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 4162 - 4168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kezic and P. G. McMenamin Differential turnover rates of monocyte-derived cells in varied ocular tissue microenvironments J. Leukoc. Biol., September 1, 2008; 84(3): 721 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gillette-Ferguson, K. Daehnel, A. G. Hise, Y. Sun, E. Carlson, E. Diaconu, H. F. McGarry, M. J. Taylor, and E. Pearlman Toll-Like Receptor 2 Regulates CXC Chemokine Production and Neutrophil Recruitment to the Cornea in Onchocerca volvulus/ Wolbachia-Induced Keratitis Infect. Immun., December 1, 2007; 75(12): 5908 - 5915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nakao, Y. Hata, M. Miura, K. Noda, Y. N. Kimura, S. Kawahara, T. Kita, T. Hisatomi, T. Nakazawa, Y. Jin, et al. Dexamethasone Inhibits Interleukin-1{beta}-Induced Corneal Neovascularization: Role of Nuclear Factor-{kappa}B-Activated Stromal Cells in Inflammatory Angiogenesis Am. J. Pathol., September 1, 2007; 171(3): 1058 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hisatomi, K.-h. Sonoda, F. Ishikawa, H. Qiao, T. Nakazawa, M. Fukata, T. Nakamura, K. Noda, S. Miyahara, M. Harada, et al. Identification of resident and inflammatory bone marrow derived cells in the sclera by bone marrow and haematopoietic stem cell transplantation Br. J. Ophthalmol., April 1, 2007; 91(4): 520 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lin, E. Carlson, E. Diaconu, and E. Pearlman CXCL1/KC and CXCL5/LIX are selectively produced by corneal fibroblasts and mediate neutrophil infiltration to the corneal stroma in LPS keratitis J. Leukoc. Biol., March 1, 2007; 81(3): 786 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ebihara, S. Yamagami, S. Yokoo, S. Amano, and A. Murakami Involvement of C-C Chemokine Ligand 2-CCR2 Interaction in Monocyte-Lineage Cell Recruitment of Normal Human Corneal Stroma J. Immunol., March 1, 2007; 178(5): 3288 - 3292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Barbaro, E. Di Iorio, S. Ferrari, L. Bisceglia, A. Ruzza, M. De Luca, and G. Pellegrini Expression of VSX1 in Human Corneal Keratocytes during Differentiation into Myofibroblasts in Response to Wound Healing Invest. Ophthalmol. Vis. Sci., December 1, 2006; 47(12): 5243 - 5250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yoshida, S. Shimmura, N. Nagoshi, K. Fukuda, Y. Matsuzaki, H. Okano, and K. Tsubota Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea Stem Cells, December 1, 2006; 24(12): 2714 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Oshima, K-H Sonoda, C Tsutsumi-Miyahara, H Qiao, T Hisatomi, S Nakao, S Hamano, K Egashira, I F Charo, and T Ishibashi Analysis of corneal inflammation induced by cauterisation in CCR2 and MCP-1 knockout mice Br. J. Ophthalmol., February 1, 2006; 90(2): 218 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Carlson, J. Drazba, X. Yang, and V. L. Perez Visualization and Characterization of Inflammatory Cell Recruitment and Migration through the Corneal Stroma in Endotoxin-Induced Keratitis Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 241 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yamagami, N. Ebihara, T. Usui, S. Yokoo, and S. Amano Bone Marrow-Derived Cells in Normal Human Corneal Stroma Arch Ophthalmol, January 1, 2006; 124(1): 62 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Espana, T. Kawakita, M. A. Di Pascuale, W. Li, L.-K. Yeh, J.-M. Parel, C.-Y. Liu, and S. C. G. Tseng The Heterogeneous Murine Corneal Stromal Cell Populations In Vitro Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4528 - 4535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Ozerdem, K. Alitalo, P. Salven, and A. Li Contribution of Bone Marrow-Derived Pericyte Precursor Cells to Corneal Vasculogenesis Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3502 - 3506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kawakita, E. M. Espana, H. He, W. Li, C.-Y. Liu, and S. C.G. Tseng Intrastromal Invasion by Limbal Epithelial Cells Is Mediated by Epithelial-Mesenchymal Transition Activated by Air Exposure Am. J. Pathol., August 1, 2005; 167(2): 381 - 393. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
