IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2005;46:1348-1355.)
© 2005 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.04-0542
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamamoto, N.
Right arrow Articles by Jester, J. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, N.
Right arrow Articles by Jester, J. V.

Internalization of Pseudomonas aeruginosa Is Mediated by Lipid Rafts in Contact Lens–Wearing Rabbit and Cultured Human Corneal Epithelial Cells

Naoka Yamamoto, Nobutaka Yamamoto, Matthew W. Petroll, H. Dwight Cavanagh, and James V. Jester

From the Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas.

PURPOSE. The internalization of Pseudomonas aeruginosa (PA) in nasal and tracheal epithelium has recently been shown to involve the formation of cholesterol- and sphingolipid-rich plasma membrane domains (lipid rafts). The purpose of this study was to investigate the role of lipid rafts in PA internalization by corneal epithelium in vivo, in vitro, and after contact lens wear.

METHODS. Lipid raft formation was evaluated in rabbit corneas with and without contact lens wear and a human corneal epithelial (hTCEpi) cell line before and after PA infection with cornea-pathogenic strains by staining with FITC-conjugated cholera toxin ß-subunit, known to bind the lipid raft component GM1. Bacterial internalization was assessed by gentamicin survival assay. The role of lipid rafts in PA internalization was evaluated by pretreatment of hTCEpi cells with cholesterol metabolism inhibitors. The interaction of PA with lipid rafts was confirmed by flow cytometry.

RESULTS. Contact lens wear in rabbits induced lipid raft formation in occasional surface corneal epithelial cells. Subsequent PA exposure showed preferential binding to lipid raft–forming cells, leading to lipid raft aggregation and PA internalization. A similar sequence of lipid raft formation and PA internalization was also observed in hTCEpi for all PA strains. Internalization of all PA strains was blocked by three cholesterol metabolism inhibitors (P < 0.01). Flow cytometry showed an association of PA with rafts.

CONCLUSIONS. These findings demonstrate that contact-lens–mediated PA internalization involves lipid raft formation. Also, hTCEpi cells may be used as an experimental model for studying further the molecular mechanism(s) of PA infection in the corneal epithelium.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
A. A. Angus, A. A. Lee, D. K. Augustin, E. J. Lee, D. J. Evans, and S. M. J. Fleiszig
Pseudomonas aeruginosa Induces Membrane Blebs in Epithelial Cells, Which Are Utilized as a Niche for Intracellular Replication and Motility
Infect. Immun., May 1, 2008; 76(5): 1992 - 2001.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Zaidi, M. Bajmoczi, T. Zaidi, D. E. Golan, and G. B. Pier
Disruption of CFTR-Dependent Lipid Rafts Reduces Bacterial Levels and Corneal Disease in a Murine Model of Pseudomonas aeruginosa Keratitis
Invest. Ophthalmol. Vis. Sci., March 1, 2008; 49(3): 1000 - 1009.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. M. Robertson, W. M. Petroll, and H. D. Cavanagh
The Effect of Nonpreserved Care Solutions on 12 Months of Daily and Extended Silicone Hydrogel Contact Lens Wear
Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 7 - 15.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. N. Atapattu and C. J. Czuprynski
Mannheimia haemolytica Leukotoxin Binds to Lipid Rafts in Bovine Lymphoblastoid Cells and Is Internalized in a Dynamin-2- and Clathrin-Dependent Manner
Infect. Immun., October 1, 2007; 75(10): 4719 - 4727.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. W. Du, F. Zhang, J. E. Capo-Aponte, S. D. Tachado, J. Zhang, F.-S. X. Yu, R. A. Sack, H. Koziel, and P. S. Reinach
AsialoGM1-Mediated IL-8 Release by Human Corneal Epithelial Cells Requires Coexpression of TLR5
Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 4810 - 4818.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. Yamamoto, N. Yamamoto, M. W. Petroll, J. V. Jester, and H. D. Cavanagh
Regulation of Pseudomonas aeruginosa Internalization after Contact Lens Wear In Vivo and in Serum-Free Culture by Ocular Surface Cells.
Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3430 - 3440.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. Zulianello, C. Canard, T. Kohler, D. Caille, J.-S. Lacroix, and P. Meda
Rhamnolipids Are Virulence Factors That Promote Early Infiltration of Primary Human Airway Epithelia by Pseudomonas aeruginosa.
Infect. Immun., June 1, 2006; 74(6): 3134 - 3147.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Association for Research in Vision and Ophthalmology