IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2005;46:1604-1614.)
© 2005 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.04-1453
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y.
Right arrow Articles by Conrad, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y.
Right arrow Articles by Conrad, G. W.

Detection and Quantification of Sulfated Disaccharides from Keratan Sulfate and Chondroitin/Dermatan Sulfate during Chick Corneal Development by ESI-MS/MS

Yuntao Zhang,1 Abigail H. Conrad,1 Elena S. Tasheva,1 Ke An,1 Lolita M. Corpuz,1 Yutaka Kariya,2 Kiyoshi Suzuki,2 and Gary W. Conrad1

1From the Division of Biology, Kansas State University, Manhattan, Kansas; and the 2Central Research Laboratories, Seikagaku Corporation, Tokyo, Japan.

PURPOSE. To identify and quantify changes in keratan sulfate (KS) and chondroitin/dermatan sulfate (CS/DS) sulfated disaccharides in the developing chick cornea using electrospray ionization tandem mass spectrometry (ESI-MS/MS).

METHODS. Cryostat sections of fresh nonfixed corneas were obtained from White Leghorn embryonic day (E)8 to E20 chicks, and from 4- and 70-week-old chickens. Tissue sections on glass slides were incubated with selected glycosidase enzymes. Digest solutions were analyzed directly by ESI-MS/MS.

RESULTS. The concentration of KS monosulfated disaccharide (MSD) Gal-ß-1,4-GlcNAc(6S) in E8 cornea equaled that at E20, declined to its lowest level by E10, increased to a second peak by E14, decreased to a second low by E18, peaked again by E20, and remained high in adult corneas. A similar concentration profile was observed for KS disulfated disaccharide (DSD) Gal(6S)-ß-1,4-GlcNAc(6S), and thus also for total sulfated KS disaccharides. The molar percent of DSD was higher than that of MSD from E8 to E18, equivalent at E20, and less than that of MSD in adult corneas. In contrast, total concentration of CS/DS {Delta}di-4S plus {Delta}di-6S decreases as development progresses and is lowest in adult corneas. Concentration and molar percent of {Delta}di-6S is highest at E8, then decreases through development as the concentration and molar percent of {Delta}di-4S increases from E8 and exceeds that of {Delta}di-6S after E14.

CONCLUSIONS. New, rapid, direct chemical analysis of extracellular matrix components obtained from sections from embryonic and adult chick corneas reveals heretofore undetected changes in sulfation characteristics of KS and CS/DS disaccharides during corneal development.




This article has been cited by other articles:


Home page
 IOVSHome page
A. H. Conrad, J. M. Strafuss, M. D. Wittman, S. Conway, and G. W. Conrad
Thyroxine Increases the Rate but Does Not Alter the Pattern of Innervation during Embryonic Chick Corneal Development
Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 139 - 153.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Zhang, I. Schmack, D. G. Dawson, H. E. Grossniklaus, A. H. Conrad, Y. Kariya, K. Suzuki, H. F. Edelhauser, and G. W. Conrad
Keratan sulfate and chondroitin/dermatan sulfate in maximally recovered hypocellular stromal interface scars of postmortem human LASIK corneas.
Invest. Ophthalmol. Vis. Sci., June 1, 2006; 47(6): 2390 - 2396.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. H. Conrad, Y. Zhang, A. R. Walker, L. A. Olberding, A. Hanzlick, A. J. Zimmer, R. Morffi, and G. W. Conrad
Thyroxine Affects Expression of KSPG-Related Genes, the Carbonic Anhydrase II Gene, and KS Sulfation in the Embryonic Chicken Cornea
Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 120 - 132.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Association for Research in Vision and Ophthalmology