HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Muñoz, M. Articles by Montero, I. Search for Related Content PubMed PubMed Citation Articles by Muñoz, M. Articles by Montero, I.

Antitumoral Action of the Neurokinin-1-Receptor Antagonist L-733,060 and Mitogenic Action of Substance P on Human Retinoblastoma Cell Lines

¹From the Hospital Universitario Virgen del Rocío, Sevilla, Spain; and ²Laboratorio de Neuroanatomía de los Sistemas Peptidérgicos, Instituto de Neurociencia de Castilla y León (INCYL), Salamanca, Spain.

PURPOSE. Activation of the neurokinin-1 receptor is known to induce proliferation in tumor cells, but it is as yet unknown whether this applies to retinoblastoma. This was an in vitro study of the growth inhibitory capacity of the potent and long-acting neurokinin-1 receptor antagonist L-733,060, at concentrations ranging from 7.5 to 20 µM, against the human retinoblastoma line WERI-Rb-1 and from 10 to 25 µM against the human retinoblastoma line Y-79. The ability of substance P (an neurokinin-1 stimulator) to activate the cell growth of these retinoblastoma cell lines was also determined.

METHODS. A cell counter was used to determine the number of viable cells, followed by application of the tetrazolium compound WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4 nitrophenyl))-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt, colorimetric method to evaluate cell viability in this cytotoxicity assay.

RESULTS. Nanomolar concentrations of substance P increased the growth of both cell lines and micromolar concentrations of L-733,060 inhibited the growth of the two cell lines studied, with and without previous administration of substance P. L-733,060 inhibited the growth of the WERI-Rb-1 and Y-79 cell lines in a dose-dependent manner. IC₅₀ was 12.15 µM for 49 hours for WERI-Rb1 and 17.38 µM for 40 hours for Y-79.

CONCLUSIONS. The findings demonstrate that substance P is a mitogen and also indicate that the neurokinin-1 receptor antagonist L-733,060 acts on both human retinoblastoma cell lines as an antitumoral agent.

This article has been cited by other articles:

				S. BRENER, M. A. GONZALEZ-MOLES, D. TOSTES, F. ESTEBAN, J. A. GIL-MONTOYA, I. RUIZ-AVILA, M. BRAVO, and M. MUNOZ A Role for the Substance P/NK-1 Receptor Complex in Cell Proliferation in Oral Squamous Cell Carcinoma Anticancer Res, June 1, 2009; 29(6): 2323 - 2329. [Abstract] [Full Text] [PDF]

				M. Munoz, M. Rosso, R. Covenas, I. Montero, M. A. Gonzalez-Moles, and M. J. Robles Neurokinin-1 Receptors Located in Human Retinoblastoma Cell Lines: Antitumor Action of Its Antagonist, L-732,138 Invest. Ophthalmol. Vis. Sci., June 1, 2007; 48(6): 2775 - 2781. [Abstract] [Full Text] [PDF]