IOVS Molecular and Cellular Biology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2006;47:249-255.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.05-0774
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hattori, T.
Right arrow Articles by Taguchi, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hattori, T.
Right arrow Articles by Taguchi, O.

Alpha-internexin as a Corneal Autoantigen in Spontaneous Autoimmune Keratitis Mouse Model

Takaaki Hattori,1,2 Masaru Takeuchi,1 Katsuhiro Ohno,2,3 Midori Ogawara,4 Tetsuya Asatani,1 Yoshihiko Usui,1 Ryuji Muramatsu,1 Masaki Inagaki,5 Masahiko Usui,1 and Osamu Taguchi2

1From the Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan; Divisions of 2Molecular Pathology and 5Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan; 3Shiroyama Park Dental Clinic, Nagoya, Japan; and 4Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

PURPOSE. The purpose of this study was to identify target antigens of autoimmune keratitis with a disease-prone mouse model.

METHODS. BALB/c nude mice grafted with embryonic rat thymi (TG nude mice) develop various organ-localized autoimmune lesions, including keratitis. A hybridoma producing a monoclonal antibody (OT-20), specific for corneal epithelium was established by using spleen cells from this model mouse of keratitis, and the target of OT-20 was identified by immunoblot analysis. Then, using the antigen, T-cell proliferation and cytokine production by TG nude mice with keratitis were examined.

RESULTS. Immunoblot analysis revealed {alpha}-internexin to be the target antigen of OT-20 that specifically recognizes corneal epithelium. Sera from TG nude mice with keratitis reacted with {alpha}-internexin on Western blot analysis, and the T cells of these mice on stimulation with {alpha}-internexin exhibited proliferation responses and produced IL-2, IFN-{gamma}, and TNF-{alpha}, but not IL-4 or IL-5.

CONCLUSIONS. These results suggest that {alpha}-internexin is one of the corneal antigens associated with keratitis, developing spontaneously in TG-nude mice, with a probable pathogenic role.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. X. Zhu, H. A. Doyle, M. J. Mamula, and D. W. Aswad
Protein Repair in the Brain, Proteomic Analysis of Endogenous Substrates for Protein L-Isoaspartyl Methyltransferase in Mouse Brain
J. Biol. Chem., November 3, 2006; 281(44): 33802 - 33813.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Association for Research in Vision and Ophthalmology