IOVS Molecular and Cellular Biology
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(Investigative Ophthalmology and Visual Science. 2006;47:4188-4193.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.05-1496
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Uncoordinated Expression of Myosin Heavy Chains and Myosin-Binding Protein C Isoforms in Human Extraocular Muscles

Daniel Kjellgren,1 Per Stål,2 Lars Larsson,3 Dieter Fürst,4 and Fatima Pedrosa-Domellöf1,2

1From the Department of Clinical Sciences, Ophthalmology, Umeå University, Umeå, Sweden; the 2Department of Integrative Medical Biology, Section of Anatomy, Umeå University, Umeå, Sweden; the 3Department of Clinical Neurophysiology, Academic Hospital, Uppsala University, Uppsala, Sweden; and the 4Institute for Biochemistry and Biology, Department of Molecular Cell Biology, University of Bonn, Bonn, Germany.

PURPOSE. To examine the distribution of myosin-binding protein C (MyBP-C) in human extraocular muscles (EOMs) and to correlate the myosin heavy chain (MyHC) and the MyBP-C composition of the fibers.

METHODS. Samples from 17 EOMs, 3 levator palpebrae (LP), and 6 limb muscles were analyzed with SDS-PAGE and immunoblot or processed for immunocytochemistry with monoclonal antibodies (mAbs) against MyBP-C-fast, MyBP-C-slow, MyHCIIa, MyHCI, MyHCsto, MyHC{alpha}-cardiac, and MyHCemb.

RESULTS. In the limb muscle samples, fast fibers were labeled with anti-MyBP-C-fast and anti-MyBP-C-slow, whereas the slow fibers were immunostained with anti-MyBP-C-slow only, in accordance with previous studies. In 11 EOM samples MyBP-C-fast was not detected, and weak staining with anti-MyBP-C-fast was seen only in a few fibers in the proximal part of 2 muscles. The mAb against MyBP-C-slow labeled all fibers, but fibers containing MyHCI were generally more strongly stained. In the levator palpebrae, immunostaining with anti-MyBP-C-fast was present in some fibers labeled with anti-MyHCIIa and/or anti-MyHCeom. MyBP-C-fast and -intermediate were not detected biochemically in the EOMs.

CONCLUSIONS. The lack of MyBP-C-fast and intermediate is an additional feature of the human EOM allotype. The true EOMs have a unique myofibrillar protein isoform composition reflecting their special structural and functional properties. The levator palpebrae muscle phenotype is intermediate between that of the EOMs and the limb muscles.






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