HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Li, Z. Articles by Smith, C. W. Search for Related Content PubMed PubMed Citation Articles by Li, Z. Articles by Smith, C. W.

Platelet Response to Corneal Abrasion Is Necessary for Acute Inflammation and Efficient Re-epithelialization

¹From the Section of Leukocyte Biology, Department of Pediatrics, Children’s Nutrition Research Center, the ⁴Section of Cardiovascular Sciences and the ³Medical Care Line, Michael E. DeBakey V.A. Medical Center, Department of Medicine, and the ⁵Children’s Nutrition Research Center, Baylor College of Medicine, Houston, Texas; and the ²Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, China.

PURPOSE. Adhesion molecules play a critical role in leukocyte emigration to wound sites, but differences are evident in different vascular beds. In this study, the contributions of P-selectin to neutrophil emigration into the cornea after central epithelial abrasion were investigated.

METHODS. Re-epithelialization, neutrophil influx, and platelet accumulation were assessed in C57BL/6 mice after removal of a 2-mm diameter area of central corneal epithelium that did not directly injure the limbal vessels or the avascular stroma of the cornea. Comparisons were made between wild-type (WT) mice and mice with targeted deletions of genes for P-selectin, CD18, or CD54, or mice with antibody-induced neutropenia or thrombocytopenia.

RESULTS. After central corneal epithelial abrasion, platelets localized in the limbal vessels and neutrophils emigrated from the limbal vessels to the region of the epithelial wound. There was temporal correspondence of platelet and neutrophil localization, peaking within 12 hours of wounding. Platelet accumulation, neutrophil emigration and corneal epithelial healing as measured by wound closure, basal epithelial cell density, and epithelial cell division were significantly reduced in P-selectin–deficient mice (P-sel^–/–). Anti-GP1b antibody–induced thrombocytopenia in WT mice significantly reduced platelet and neutrophil accumulation and wound healing. Passive transfer of wild-type platelets into P-sel^–/– mice significantly restored platelet localization in limbal vessels, neutrophil emigration, epithelial cell division, and epithelial cell migration into the abraded region of the cornea.

CONCLUSIONS. Platelet localization in the limbus of abraded corneas contributes to re-epithelialization, and P-selectin provides a necessary step in this process.

This article has been cited by other articles:

				K. Gronert Lipid Autacoids in Inflammation and Injury Responses: A Matter of Privilege Mol. Interv., February 1, 2008; 8(1): 28 - 35. [Abstract] [Full Text] [PDF]

				M. S. Petrescu, C. L. Larry, R. A. Bowden, G. W. Williams, D. Gagen, Z. Li, C. W. Smith, and A. R. Burns Neutrophil Interactions with Keratocytes during Corneal Epithelial Wound Healing: A Role for CD18 Integrins Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 5023 - 5029. [Abstract] [Full Text] [PDF]

				Z. Li, A. R. Burns, R. E. Rumbaut, and C. W. Smith {gamma}{delta} T Cells Are Necessary for Platelet and Neutrophil Accumulation in Limbal Vessels and Efficient Epithelial Repair after Corneal Abrasion Am. J. Pathol., September 1, 2007; 171(3): 838 - 845. [Abstract] [Full Text] [PDF]