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(Investigative Ophthalmology and Visual Science. 2006;47:5177-5180.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.06-0332
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Correlation of Heterogeneity for Chromosome 3 Copy Number with Cell Type in Choroidal Melanoma of Mixed-Cell Type

Teresa Sandinha,1 Maura Farquharson,2 Ian McKay,3 and Fiona Roberts4

1From the Tennent Institute of Ophthalmology, Gartnavel General Hospital, Glasgow, Scotland, United Kingdom; the 2University Department of Pathology, Royal Infirmary, Glasgow, Scotland, United Kingdom; the 3Department of Microbiology, University of Glasgow, Glasgow, Scotland, United Kingdom; and the 4University Department of Pathology, Western Infirmary, Glasgow, Scotland, United Kingdom.

PURPOSE. To study heterogeneity for chromosome 3 copy number in mixed choroidal melanoma with discrete populations of spindle and epithelioid cells using chromosome in situ hybridization (CISH) and to correlate chromosomal loss with cell type.

METHODS. Twenty-two archival cases of choroidal melanoma with discrete populations of spindle and epithelioid cells were identified. CISH was used to identify chromosome 3 copy number in spindle and epithelioid areas.

RESULTS. Monosomy 3 was detected in 12 (55%) of 22 choroidal melanomas. Of these, 10 (45%) had two copies of chromosome 3 in both epithelioid and spindle cells, 7 (32%) showed monosomy 3 in the epithelioid areas only, and 5 (23%) showed monosomy 3 in both epithelioid and spindle areas.

CONCLUSIONS. CISH is a useful technique for analyzing chromosome copy number in different cell populations within a tumor. In mixed choroidal melanomas with discrete spindle and epithelioid cell populations, there may be heterogeneity for chromosome 3 copy number that correlates with areas of different cell type.




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