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(Investigative Ophthalmology and Visual Science. 2006;47:5243-5250.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.06-0185
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Expression of VSX1 in Human Corneal Keratocytes during Differentiation into Myofibroblasts in Response to Wound Healing

Vanessa Barbaro,1,2 Enzo Di Iorio,1,2 Stefano Ferrari,1 Luigi Bisceglia,3 Alessandro Ruzza,1 Michele De Luca,1,4 and Graziella Pellegrini1,4

1From the Epithelial Stem Cell Research Centre, Veneto Eye Bank Foundation, Venice, Italy; the 3Medical Genetics Service, IRCCS-CSS (Istituto Ricerca e Cura a Carattere Scientifico-Casa Sollievo della Sofferenza) Hospital, San Giovanni Rotondo (FG), Italy; and the 4Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy.

PURPOSE. To characterize the expression of the visual system homeobox gene (VSX1) in human corneal keratocytes both in vitro and in vivo.

METHODS. The expression of VSX1 was evaluated through semiquantitative RT-PCR, immunofluorescence and in situ hybridization both in corneas (either freshly obtained or wounded) and in collagenase/hyaluronidase-isolated keratocytes grown in the absence or presence of serum to promote keratocyte-to-myofibroblast differentiation.

RESULTS. Quiescent or resting keratocytes normally residing in the corneal stroma or cultured in vitro in the absence of serum did not express VSX1. In wounded corneas or when cultured in the presence of serum to mimic wound-healing responses, keratocytes underwent fibroblastic transformation (with appearance of {alpha}-SMA and disappearance of CD-34 and keratocan signals) and started expressing VSX1.

CONCLUSIONS. The results show that VSX1 is expressed in vitro and in vivo during human corneal wound healing, a process in which differentiation of corneal keratocytes into myofibroblasts occurs. These data may help to elucidate the role of VSX1 in cornea physiology suggesting a potential involvement in cornea-related diseases such as keratoconus.






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Copyright © 2006 by the Association for Research in Vision and Ophthalmology