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(Investigative Ophthalmology and Visual Science. 2006;47:552-557.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.05-0995
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Transplantation of Tissue-Engineered Epithelial Cell Sheets after Excimer Laser Photoablation Reduces Postoperative Corneal Haze

Yasutaka Hayashida,1 Kohji Nishida,1 Masayuki Yamato,2 Joseph Yang,2 Hiroaki Sugiyama,1 Katsuhiko Watanabe,1 Yuichi Hori,1 Naoyuki Maeda,1 Akihiko Kikuchi,2 Teruo Okano,2 and Yasuo Tano1

1From the Department of Ophthalmology, Osaka University Medical School, Osaka, Japan; and the 2Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan.

PURPOSE. To apply tissue-engineered cell sheet transplantation after excimer laser keratectomy as a novel approach for the reduction of postoperative corneal haze.

METHODS. Limbal biopsy specimens were obtained, and epithelial cells were cultured on temperature-responsive culture inserts without the use of feeder cells. Laser keratectomy (7.0-mm ablation zone and 160-µm depth) was performed in the contralateral eye, and autologous epithelial cell sheets were transplanted to the ablated corneal stroma. Transplant and control group eyes were assessed by slit lamp biomicroscopy, and corneal haze was scored in a masked fashion, according to the Fantes grading scale. For further examination histologic and immunohistochemical analyses were performed.

RESULTS. Tissue-engineered cell sheets produced stable attachment to the laser-ablated sites, resulting in epithelialization, 5 minutes after transplantation. Conversely, control corneas required 3 to 5 days for complete re-epithelialization. At both 1 and 2 months after surgery, corneal haze was significantly inhibited in the transplant group. Histologic analyses showed that the number of keratocytes undergoing apoptosis was decreased in the transplant group at 3 days after surgery. Similarly, the expression of both collagen III and {alpha}-smooth muscle actin, which may enhance corneal haze, were diminished in the transplant group at 2 months.

CONCLUSIONS. The transplantation of tissue-engineered epithelial cell sheets can successfully prevent the development of corneal haze after excimer laser keratectomy.






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Copyright © 2006 by the Association for Research in Vision and Ophthalmology