IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2006;47:823-830.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.05-0902
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ito, T.
Right arrow Articles by Nakazawa, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ito, T.
Right arrow Articles by Nakazawa, M.

Effects of Antiglaucoma Drops on MMP and TIMP Balance in Conjunctival and Subconjunctival Tissue

Tadashi Ito, Hiroshi Ohguro, Kazuhisa Mamiya, Ikuyo Ohguro, and Mitsuru Nakazawa

From the Department of Ophthalmology, Hirosaki University School of Medicine, Hirosaki, Japan.

PURPOSE. To study the effects of antiglaucoma drugs on metabolism within the extracellular matrix (ECM) of the ocular surface, including corneal, conjunctival, and subconjunctival tissue.

METHOD. Several antiglaucoma drugs—including ß-blockers, {alpha}/ß-blockers, {alpha}1-blocker, {alpha}2-agonist, and prostaglandin derivative—were topically administrated to rat eyes daily for 2 weeks or were incubated with human corneal cells or human fibroblasts for 72 hours. Thereafter, expression and enzymatic activity of the matrix metalloproteinases (MMPs), a group of enzymes proteolyzing ECM and their inhibitors, called tissue inhibitors of metalloproteinase (TIMPs), were evaluated.

RESULTS. Quantitative RT-PCR revealed significantly upregulated and downregulated expression of MMPs and TIMPs, respectively, in rat conjunctival and subconjunctival tissue on the administration of {alpha}/ß-blockers, {alpha}1-blocker, {alpha}2-agonist, and prostaglandin derivative, suggesting that these drugs may enhance ECM degradation. However, in contrast, ß-blocker administration caused reverse effects—that is, upregulation and downregulation of TIMPs and MMPs, respectively. Enzymatic activity of MMPs in rat conjunctival and subconjunctival tissue analyzed by biochemical assay and zymography was markedly enhanced on the administration of {alpha}/ß-blockers, {alpha}1-blocker, {alpha}2-agonist, and prostaglandin derivative, but not of ß-blockers. Similar effects of these antiglaucoma drugs were observed in cultured human corneal cells and human fibroblast cells.

CONCLUSIONS. The present experimental observations suggest that some {alpha}/ß-blockers, {alpha}1-blocker, {alpha}2-agonist, and prostaglandin derivative stimulate ECM degradation of ocular surface tissue by modulating the balance between MMPs and TIMPs.




This article has been cited by other articles:


Home page
 Br J OphthalmolHome page
N Terai, U Schlotzer-Schrehardt, J Lampel, A G Bohm, C Rummelt, E Schmidt, and L E Pillunat
Effect of latanoprost and timolol on the histopathology of the human conjunctiva
Br J Ophthalmol, February 1, 2009; 93(2): 219 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
F. W. Rozsa, K. Scott, H. Pawar, S. Moroi, and J. E. Richards
Effects of Timolol on MYOC, OPTN, and WDR36 RNA Levels
Arch Ophthalmol, January 1, 2008; 126(1): 86 - 93.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
M. C. Grieshaber, A. Schoetzau, C. Zawinka, J. Flammer, and S. Orgul
Effect of Central Corneal Thickness on Dynamic Contour Tonometry and Goldmann Applanation Tonometry in Primary Open-angle Glaucoma
Arch Ophthalmol, June 1, 2007; 125(6): 740 - 744.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Association for Research in Vision and Ophthalmology