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(Investigative Ophthalmology and Visual Science. 2006;47:1269-1273.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.05-1321
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Immunohistochemical Detection of Multidrug-Resistant Protein Expression in Retinoblastoma Treated by Primary Enucleation

Matthew W. Wilson,1,2 Charles H. Fraga,3 Christine E. Fuller,4 Carlos Rodriguez-Galindo,5 John Mancini,5 Nikolaus Hagedorn,3 Markos L. Leggas,3 and Clinton F. Stewart3

1From the Department of Ophthalmology, University of Tennessee Health Science Center, Memphis, Tennessee; and the 2Departments of Surgery, 3Pharmaceutical Sciences, 4Pathology, and 5Hematology/Oncology, St. Jude Children’s Research Hospital, Memphis, Tennessee.

PURPOSE. To compare the expression of multidrug-resistant proteins in retinoblastoma tumors among eyes treated with primary enucleation.

METHODS. A group of 18 patients with unilateral retinoblastoma with advanced intraocular disease was selected for the study. All patients had undergone primary enucleation. A histologic specimen from each patient was retrieved from the pathology archives and a tissue gene microarray was constructed (0.6 x 3–4 mm). Standard immunohistochemical techniques were used to study the tissue microarrays for the expression of the ATP-binding cassette (ABC) transporters: breast cancer resistance protein (BCRP; ABCG2), multidrug-resistant protein 1/P-glycoprotein (MDR1/Pgp; ABCB1), multidrug-resistant–associated protein 1 (MRP1; ABCC1), MRP2 (ABCC2), and MRP4 (ABCC4).

RESULTS. Of the 18 specimens retrieved, 16 had adequate tissue for study. MRP1 was expressed in 8 (50%) of 16 tumors, and MRP2 was expressed in 5 (31%) of 16 tumors. MDR1/Pgp was found in 2 (12%) of 16 retinoblastomas. MRP4 and BCRP were not detected in any of the tumors studied.

CONCLUSIONS. The results show that multiple ABC transporters were present in a cohort of sporadic patients with unilateral retinoblastoma who underwent primary enucleation. Studies are planned of the expression of ABC transporters in eyes treated by chemotherapy and/or radiation as a comparison with this group.




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