IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2006;47:2390-2396.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.05-1559
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y.
Right arrow Articles by Conrad, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y.
Right arrow Articles by Conrad, G. W.

Keratan Sulfate and Chondroitin/Dermatan Sulfate in Maximally Recovered Hypocellular Stromal Interface Scars of Postmortem Human LASIK Corneas

Yuntao Zhang,1,2 Ingo Schmack,2,3,4 Daniel G. Dawson,2,3 Hans E. Grossniklaus,3 Abigail H. Conrad,1 Yutaka Kariya,5 Kiyoshi Suzuki,5 Henry F. Edelhauser,3 and Gary W. Conrad1

1From the Division of Biology, Kansas State University, Manhattan, Kansas; the 3Department of Ophthalmology, Emory University, Atlanta, Georgia; the 4Department of Ophthalmology, Ruprecht-Karls-University, Heidelberg, Germany; and the 5Central Research Laboratories, Seikagaku Corp., Higashiyamato-shi, Japan.

PURPOSE. To analyze the amounts and distributions of nonsulfated and sulfated keratan sulfate (KS) and chondroitin/dermatan sulfate (CS/DS) disaccharides in the interface wound of human postmortem LASIK corneas in comparison with normal control corneas.

METHODS. Corneal stromal tissue samples from central and paracentral hypocellular primitive stromal interface scars of human LASIK corneas and from similar regions of normal control corneas were collected by laser capture microdissection (LCM) and subsequently were digested with specific glycosidase enzymes. Digests were directly analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS).

RESULTS. Concentrations of both monosulfated GlcNAc(6S)-ß-1,3-Gal (MSD2) and disulfated Gal (6S)-ß-1,4-GlcNAc(6S) (DSD) KS disaccharides from the LASIK interface scars were significantly lower than in normal control corneal stromas. No significant difference was found for the concentration of nonsulfated (NSD) KS disaccharides in LASIK interface scars compared with normal controls. The concentration of {Delta}UA-ß-1,3-GalNAc(6S) ({Delta}di-6S) CS/DS disaccharides from the LASIK interface scar was significantly higher than normal corneal stroma, whereas concentrations of {Delta}UA-ß-1,3-GalNAc(4S) ({Delta}di-4S) and nonsulfated {Delta}di-0S CS/DS disaccharides demonstrated no significant differences from normal corneas.

CONCLUSIONS. The profiles of KS and CS/DS disaccharides in LASIK interface scars are significantly different from those in normal cornea stromal tissue, as revealed by LCM and ESI-MS/MS.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Association for Research in Vision and Ophthalmology