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Inhibitory Effects of Lutein on Endotoxin-Induced Uveitis in Lewis Rats

¹From the Departments of Ophthalmology and Visual Sciences and ³Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Japan; and the ²Department of Clinical Pharmaceutics and Therapeutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

PURPOSE. Lutein deposits in the macula and lens of human eyes with high concentration and is well known as an eye-protective nutrient for its beneficial effects on eye disease such as age-related macular degeneration and cataract. The purpose of the present study was to investigate the effects of lutein on endotoxin-induced uveitis (EIU) in rats.

METHODS. EIU was induced in male Lewis rats by subcutaneous injection of 200 µg lipopolysaccharide. Lutein or dexamethasone was administered intravenously at 30 minutes before, at the same time as, and at 30 minutes after LPS treatment. The aqueous humor was collected at 24 hours after LPS injection, the number of infiltrating cells, the protein concentration, and the levels of nitric oxide (NO), tumor necrosis factor (TNF)-, interleukin (IL)-6, prostaglandin (PG)-E2, monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein (MIP)-2 in the aqueous humor were determined. Immunohistochemical staining with a monoclonal antibody against activated nuclear factor (NF)-B was performed to evaluate the effect of lutein on NF-B activation in the iris-ciliary body (ICB) of rats. A mouse macrophage cell line (RAW264.7 cells) was stimulated with LPS in the presence or absence of lutein. Expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and degradation of inhibitor B (IB) were analyzed by Western blot analysis.

RESULTS. Lutein suppressed the development of EIU in a dose-dependent fashion. The anti-inflammatory effect of 100 mg/kg lutein was as strong as that of 1 mg/kg dexamethasone. Treatment with lutein reduced the concentrations of NO, TNF-, IL-6, PGE2, MCP-1, and MIP-2 in aqueous humor. Lutein also suppressed the activation of NF-B in the ICB as well as iNOS and COX-2 expression and IB degradation in RAW cells.

CONCLUSIONS. These findings indicate that lutein has anti-inflammatory effects on EIU by inhibiting the NF-B dependent signaling pathway and the subsequent production of proinflammatory mediators.

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