IOVS British Journal of Pharmacology
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(Investigative Ophthalmology and Visual Science. 2006;47:2840-2846.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.05-1367
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Development of a Three-Dimensional Organ Culture Model for Corneal Wound Healing and Corneal Transplantation

Bojun Zhao,1 Leanne J. Cooper,1 Arun Brahma,2 Sheila MacNeil,3,4 Stephen Rimmer,5 and Nigel J. Fullwood1

1From Biomedical Sciences, Department of Biological Sciences, Lancaster University, Lancaster, United Kingdom; 2Manchester Royal Eye Hospital, Manchester, United Kingdom; 3Division of Clinical Sciences, Northern General Hospital, Sheffield, United Kingdom; 4Department of Engineering Materials, Kroto Research Institute, and 5The Polymer and Biomaterials Chemistry Laboratories, Department of Chemistry, University of Sheffield, Sheffield, United Kingdom.

PURPOSE. To develop and evaluate a three-dimensional organ culture system of the cornea anterior chamber that could replicate the in vivo processes occurring during corneal wound healing and corneal transplantation.

METHODS. Bovine corneoscleral buttons were clamped in a specially designed chamber through the sclera outside the limbus. The epithelium was exposed to air, and its anterior surface was automatically irrigated. The endothelial layer was perfused separately with media under normal intraocular pressure. Wound healing and corneal transplantation were observed using light, scanning, and transmission electron microscopy.

RESULTS. The organ culture system maintained the epithelium, the putative epithelial stem cells in the limbus, the stroma, and the endothelium in good condition for the 10-day period during which the system was evaluated. The authors observed that the processes of wound healing and corneal transplantation in the model appeared similar to those occurring in vivo.

CONCLUSIONS. In vitro model closely replicated the in vivo processes of wound healing and corneal transplantation. The authors believe this model will be useful for basic investigations into the cornea, such as study of the response of the cornea to surgery, wound healing, toxins, and therapeutic agents.




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B. Zhao, S. L. Allinson, A. Ma, A. J. Bentley, F. L. Martin, and N. J. Fullwood
Targeted Cornea Limbal Stem/Progenitor Cell Transfection in an Organ Culture Model
Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3395 - 3401.
[Abstract] [Full Text] [PDF]


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