IOVS JNCI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2007;48:4753-4758.)
© 2007 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.07-0433
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berkowitz, B. A.
Right arrow Articles by Gradianu, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berkowitz, B. A.
Right arrow Articles by Gradianu, M.

Impaired Apparent Ion Demand in Experimental Diabetic Retinopathy: Correction by Lipoic Acid

Bruce A. Berkowitz,1,2 Robin Roberts,1 Ann Stemmler,3 Hongmei Luan,1 and Marius Gradianu1

1From the Departments of Anatomy and Cell Biology, 2Ophthalmology, and 3Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan.

PURPOSE. To test the hypothesis that early in the course of diabetes, apparent ion demand within the retina is impaired and may be corrected by {alpha}-lipoic acid (LPA), a drug that inhibits vascular histopathology.

METHODS. Intraretinal manganese ion uptake and retinal thickness were measured from high-resolution manganese-enhanced MRI (MEMRI) data of control and streptozocin diabetic male Sprague–Dawley (SD) rats and of control and diabetic female Lewis rats with and without treatment with LPA. In a subgroup of male SD rats, blood-retinal barrier (BRB) integrity was also assessed with dynamic contrast-enhanced MRI. In addition, ion-coupled plasma-mass spectrometry (ICP-MS) was used to measure baseline whole manganese levels from retinas of control and diabetic rats.

RESULTS. Manganese ion uptake by receptor and postreceptor retina was subnormal in each untreated diabetic groups, and these deficiencies could be corrected with LPA treatment. ICP-MS studies found no differences in baseline retinal manganese concentration between control and diabetic rats. In 3-month-old diabetic male SD rats, total and postreceptor retinal thickness increased (P < 0.05) without loss of BRB integrity. In contrast, in untreated and treated diabetic female Lewis rats, retinal thicknesses were normal.

CONCLUSIONS. The present results support the hypothesis that LPA can correct the impaired apparent ion demand in experimental diabetic retinopathy.




This article has been cited by other articles:


Home page
 IOVSHome page
B. A. Berkowitz, M. Gradianu, D. Bissig, T. S. Kern, and R. Roberts
Retinal Ion Regulation in a Mouse Model of Diabetic Retinopathy: Natural History and the Effect of Cu/Zn Superoxide Dismutase Overexpression
Invest. Ophthalmol. Vis. Sci., May 1, 2009; 50(5): 2351 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. J. Calkins, P. J. Horner, R. Roberts, M. Gradianu, and B. A. Berkowitz
Manganese-Enhanced MRI of the DBA/2J Mouse Model of Hereditary Glaucoma
Invest. Ophthalmol. Vis. Sci., November 1, 2008; 49(11): 5083 - 5088.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Association for Research in Vision and Ophthalmology