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(Investigative Ophthalmology and Visual Science. 2007;48:5229-5242.)
© 2007 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.07-0122
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Comparison between the Gene Expression Profile of Human Müller Cells and Two Spontaneous Müller Cell Lines

Caroline B. Lupien,1,2 Carl Bolduc,3 Solange Landreville,1,2 and Christian Salesse1,2

1From Unité de Recherche en Ophtalmologie, 2Département d’ORLO, Faculté de Médecine, and 3Laboratoire de Recherche en Endocrinologie Moléculaire et Oncologie, Centre de Recherche du CHUQ and Université Laval, Ste-Foy, Québec, Canada.

PURPOSE. Müller cells are the principal glial cells in the retina. They play important functions in this tissue. Alterations in Müller cell behavior were observed in the retinal tissue of patients with proliferative diabetic retinopathy. The purpose of this study was to determine the gene expression profile of normal human Müller cells (NHMCs) and to compare it with that of two spontaneously generated human Müller cell lines (HMCLs) from type 1 and type 2 diabetic donors by using serial analysis of gene expression (SAGE).

METHODS. Approximatively 50,000 tags were sequenced for each SAGE library. Identification of the transcripts was obtained by matching the 15-bp tags with the UniGene and GenBank databases. Classification of the genes was based on the updated information of the genome directory found at the National Center for Biotechnology Information (NCBI) Web site.

RESULTS. SAGE was used to characterize the entire transcriptome of human Müller cells and to compare these data with those from Müller cell lines generated from type 1 and type 2 diabetic donors. The transcriptome of NHMCs and HMCLs demonstrated that "metabolism" and "protein synthesis" are the two main categories of genes expressed by human Müller cells. Only 106 genes are differentially expressed between NHMCs and HMCLs.

CONCLUSIONS. The SAGE libraries reported in this article provide the gene expression profile of NHMCs and HMLCs. It thus represents a valuable source of information regarding the function of Müller cells as well as their role in the development of diabetic retinopathy.






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Copyright © 2007 by the Association for Research in Vision and Ophthalmology