IOVS Journal of Biological Chemistry
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(Investigative Ophthalmology and Visual Science. 2007;48:5464-5472.)
© 2007 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.07-0093
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Circulating Hematopoietic Stem Cells in Patients with Neovascular Age-Related Macular Degeneration

Yuko Yodoi,1,2 Manabu Sasahara,1,2 Takanori Kameda,1 Nagahisa Yoshimura,1 and Atsushi Otani1

1From the Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan.

PURPOSE. Circulating hematopoietic stem cells (HSCs) appear to have roles in the formation of choroidal neovascularization (CNV) in age-related macular degeneration (AMD). This study was conducted to investigate whether the number or function of HSCs plays a role in neovascular AMD.

METHODS. Eighty-one patients with neovascular AMD who underwent comprehensive fundus examinations every 3 months were included. The number of CD34+ HSCs isolated from peripheral blood was counted by flow cytometry. Serum cytokine levels were assessed by enzyme-linked immunosorbent assay. To examine the function of circulating HSCs, mononuclear cells were cultured and then colony forming unit (CFU-EC) and migration were measured.

RESULTS. The number of circulating CD34+ HSCs was significantly increased in the patients with active CNV without major systemic diseases (stable: 3.8 ± 0.3 cells/µL, active: 5.5 ± 0.7 cells/µL, stable versus active: P < 0.05). The number of HSCs correlated positively with the erythropoietin serum level (r = 0.47, P = 0.002). Although there was no significant difference in the CFU-EC between the patients with CNV and the control subjects, a significant decrease of CFU-EC was observed in the patients with bilateral or larger CNV.

CONCLUSIONS. The findings suggest that CD34+ HSCs may be recruited from bone marrow through a signal from active CNV. Furthermore, HSCs may play a role in the severity of CNV.






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Copyright © 2007 by the Association for Research in Vision and Ophthalmology