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(Investigative Ophthalmology and Visual Science. 2007;48:901-906.)
© 2007 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.06-0824
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Tyrosine Phosphorylation of cGMP-Gated Ion Channels Is under Circadian Control in Chick Retina Photoreceptors

Kwon-Seok Chae,1,2 Gladys Y.-P. Ko,2,3 and Stuart E. Dryer4

1From the School of Life Sciences and Biotechnology, Korea University, Seoul, Korea; the 3Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas; and the 4Department of Biology and Biochemistry, University of Houston, Houston, Texas.

PURPOSE. To investigate the role of tyrosine phosphorylation in circadian regulation of cGMP-gated cation channels (CNGCs) of chicken cone photoreceptors.

METHODS. Chick retinas were studied on the second day of constant darkness (DD) after several days of entrainment to 12:12 hr light–dark (LD) cycles in vitro. Inside-out patch recordings were made during the subjective day and subjective night to quantify circadian changes in the sensitivity of CNGCs to activation by cGMP after treatment with various tyrosine kinase and tyrosine phosphatase inhibitors. Immunoprecipitation and immunoblot analysis were also used to examine tyrosine phosphorylation of CNGCs and closely associated proteins after separation by conventional and two-dimensional SDS-PAGE.

RESULTS. Treatment with tyrosine kinase inhibitors caused a significant decrease in K1/2 for cGMP activation of CNGCs in patches excised from cones during the subjective day, but had no effect on K1/2 during the subjective night. Conversely, treatment with a tyrosine phosphatase inhibitor caused a significant increase in the K1/2 of CNGCs in patches excised during the subjective night but had no effect on channel K1/2 during the subjective day. Broad spectrum serine-threonine phosphatase inhibitors had no effect. An 85-kDa tyrosine polypeptide that coimmunoprecipitated with CNGC {alpha}-subunits was detectable at higher levels during the subjective day than during the subjective night. CNGC {alpha}-subunits were not tyrosine phosphorylated as a function of the time of day.

CONCLUSIONS. Circadian control of cone CNGCs appears to entail elevated daytime tyrosine phosphorylation of an ~85-kDa auxiliary protein or another subunit of the CNGCs.




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 J. Neurosci.Home page
S.-K. Chen, G. Y.-P. Ko, and S. E. Dryer
Somatostatin Peptides Produce Multiple Effects on Gating Properties of Native Cone Photoreceptor cGMP-Gated Channels That Depend on Circadian Phase and Previous Illumination
J. Neurosci., November 7, 2007; 27(45): 12168 - 12175.
[Abstract] [Full Text] [PDF]


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