HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Morishige, N. Articles by Jester, J. V. Search for Related Content PubMed PubMed Citation Articles by Morishige, N. Articles by Jester, J. V.

Second-Harmonic Imaging Microscopy of Normal Human and Keratoconus Cornea

¹From the The Eye Institute, School of Medicine, University of California Irvine Medical Center, Orange, California; and the ²Department of Ophthalmology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.

PURPOSE. The purpose of this study was to evaluate the ability of second-harmonic imaging to identify differences in corneal stromal collagen organization between normal human and keratoconus corneas.

METHODS. Six normal corneas from eye bank donors and 13 corneas of patients with keratoconus, obtained after penetrating keratoplasty were examined. A femtosecond titanium-sapphire laser with 800-nm output was used to generate second-harmonic signals collected at 400 nm from central and paracentral corneal tissue blocks. Three-dimensional (3-D) data sets were collected and reconstructed to evaluate the location and orientation of stromal collagen lamellae.

RESULTS. Imaging of second-harmonic signals combined with 3-D reconstruction of the normal cornea identified a high degree of lamellar interweaving, particularly in the anterior cornea. Of note was the detection of lamellae that inserted into Bowman’s layer and were oriented transverse to the corneal surface, penetrating posteriorly approximately 120 µm. In keratoconus corneas, imaging second-harmonic signals identified less lamellar interweaving and a marked reduction or loss of lamellae inserting into Bowman’s layer in 12 of 13 cases, particularly in regions associated with cone development without breaks in Bowman’s layer or scarring.

CONCLUSIONS. Compared with normal adult corneas, marked abnormalities were detected in the organization of the anterior corneal collagen lamellae of keratoconus corneas by second harmonic imaging. These structural abnormalities are consistent with the known changes in collagen organization and biomechanical strength of keratoconus.

This article has been cited by other articles:

				N. Morishige, N. Yamada, S. Teranishi, T.-i. Chikama, T. Nishida, and A. Takahara Detection of Subepithelial Fibrosis Associated with Corneal Stromal Edema by Second Harmonic Generation Imaging Microscopy Invest. Ophthalmol. Vis. Sci., July 1, 2009; 50(7): 3145 - 3150. [Abstract] [Full Text] [PDF]

				M. Farid, N. Morishige, L. Lam, A. Wahlert, R. F. Steinert, and J. V. Jester Detection of Corneal Fibrosis by Imaging Second Harmonic-Generated Signals in Rabbit Corneas Treated with Mitomycin C after Excimer Laser Surface Ablation Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4377 - 4383. [Abstract] [Full Text] [PDF]

				J. H. Mathew, J. P. G. Bergmanson, and M. J. Doughty Fine Structure of the Interface between the Anterior Limiting Lamina and the Anterior Stromal Fibrils of the Human Cornea Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 3914 - 3918. [Abstract] [Full Text] [PDF]