HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Ruggeri, M. Articles by Puliafito, C. A. Search for Related Content PubMed PubMed Citation Articles by Ruggeri, M. Articles by Puliafito, C. A.

In Vivo Three-Dimensional High-Resolution Imaging of Rodent Retina with Spectral-Domain Optical Coherence Tomography

From the Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida.

PURPOSE. To demonstrate the application of high-resolution spectral-domain optical coherence tomography (SD-OCT) for three-dimensional (3D) retinal imaging of small animals and quantitative retinal information extraction using 3D segmentation of the OCT images.

METHODS. A high-resolution SD-OCT system was built for in vivo imaging of rodent retina. OCT fundus images similar to those acquired with a scanning laser ophthalmoscope (SLO) were constructed from the measured OCT data, which provided precise spatial registration of the OCT cross-sectional images on the fundus. A 3D segmentation algorithm was developed for calculation of the retinal thickness map. OCT images were compared by histologic examination.

RESULTS. High-quality OCT images of the retinas of mice (B6/SJLF2 for normal retina, rhodopsin-deficient Rho^–/– for photoreceptor degeneration, and LH_BETAT_AG for retinoblastoma) and rat (Wistar) were acquired. The OCT images compared well with histology. Not only was a 3D image of the tumor in a retinoblastoma mouse model successfully imaged in vivo but the tumor volume was extracted from the 3D image. Retinal thickness maps were calculated that enabled successful quantitative comparison of the retinal thickness distribution between the normal (202.3 ± 9.3 µm) and the degenerative (102.7 ± 12.6 µm) mouse retina.

CONCLUSIONS. High-resolution spectral-domain OCT provides unprecedented high-quality 2D and 3D in vivo visualization of retinal structures of mouse and rat models of retinal diseases. With the capability of 3D quantitative information extraction and precise spatial registration, the OCT system made possible longitudinal study of ocular diseases that has been impossible to conduct.

This article has been cited by other articles:

				J. Fingler, C. Readhead, D. M. Schwartz, and S. E. Fraser Phase-Contrast OCT Imaging of Transverse Flows in the Mouse Retina and Choroid Invest. Ophthalmol. Vis. Sci., November 1, 2008; 49(11): 5055 - 5059. [Abstract] [Full Text] [PDF]

				U. E. K. Wolf-Schnurrbusch, V. Enzmann, C. K. Brinkmann, and S. Wolf Morphologic Changes in Patients with Geographic Atrophy Assessed with a Novel Spectral OCT-SLO Combination Invest. Ophthalmol. Vis. Sci., July 1, 2008; 49(7): 3095 - 3099. [Abstract] [Full Text] [PDF]