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(Investigative Ophthalmology and Visual Science. 2007;48:3038-3042.)
© 2007 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.06-1309
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Influence of Thyroid Hormone on Thyroid Hormone Receptor ß-1 Expression and Lacrimal Gland and Ocular Surface Morphology

Ana Carolina Dias,1 Carolina Maria Módulo,1 Angélica Gobbi Jorge,1 Alexandre Martins Braz,1 Alceu A. Jordão, Jr,2 Rubens Bertazolli Filho,1 Jayter Silva de Paula,1 and Eduardo Melani Rocha1

1From the Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, and 2Departamento de Clinica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.

PURPOSE. Hormone diseases induce changes in the lacrimal gland (LG) and ocular surface (OS). Thyroid hormone (TH) induces cell proliferation and lipid metabolism through the activation of TH receptors. The aim of the present study was to evaluate the location and comparative expression of TH receptor ß-1 (Thrb) in LG of rats with hypothyroidism and in controls and to evaluate the impact of this disease on LG and OS structure and function.

METHODS. Hypothyroidism was induced in Wistar male rats by the long-term use of tiamazole. Ten weeks later corneal cells were collected for impression cytology (IC). Rats were humanely killed, and tissues were evaluated by immunoperoxidase staining and Western blot for Thrb. The content of malondialdehyde (MDA) and acetylcholine (ACh) in LG was determined by spectrophotometry (n = 5/group in all experiments).

RESULTS. LG weight was significantly lower in hypothyroid rats (P < 0.05). Western blot analysis indicated that LGs express Thrb and that hypothyroidism induces a higher expression of this receptor. IC was significantly different and ACh was significantly lower in hypothyroid rats (P < 0.05).

CONCLUSIONS. Chronically reduced levels of TH lead to biochemical and structural changes and modulate the levels of Thrb in LG. These events confirm that LG is a target organ for TH and may facilitate understanding of the mechanism related to dry eye in hypothyroidism.






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Copyright © 2007 by the Association for Research in Vision and Ophthalmology